Myeloid-derived suppressor cells are involved in lysosomal acid lipase deficiency-induced endothelial cell dysfunctions

Ting Zhao, Xinchun Ding, Hong Du, Cong Yan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The underlying mechanisms that lysosomal acid lipase (LAL) deficiency causes infiltration of myeloid-derived suppressor cells (MDSCs) in multiple organs and subsequent inflammation remain incompletely understood. Endothelial cells (ECs), lining the inner layer of blood vessels, constitute barriers regulating leukocytes transmigration to the site of inflammation. Therefore, we hypothesized that ECs are dysfunctional in LAL-deficient (lal-/-) mice. We found that Ly6G+ cells transmigrated more efficiently across lal -/- ECs than wild-type (lal+/+) ECs, which were associated with increased levels of PECAM-1 and MCP-1 in lal-/- ECs. In addition, lal-/- ECs showed enhanced migration and proliferation, decreased apoptosis, but impaired tube formation and angiogenesis. lal -/- ECs also suppressed T cell proliferation in vitro. Interestingly, lal-/- Ly6G+ cells promoted in vivo angiogenesis (including a tumor model), EC tube formation, and proliferation. Finally, the mammalian target of rapamycin (mTOR) pathway was activated in lal-/- ECs, and inhibition of mTOR reversed EC dysfunctions, including decreasing Ly6G+ cell transmigration, delaying migration, and relieving suppression of T cell proliferation, which was mediated by decreasing production of reactive oxygen species. Our results indicate that LAL regulates EC functions through interaction with MDSCs and modulation of the mTOR pathway, which may provide a mechanistic basis for targeting MDSCs or mTOR to rejuvenate EC functions in LAL deficiency-related diseases.

Original languageEnglish
Pages (from-to)1942-1953
Number of pages12
JournalJournal of Immunology
Volume193
Issue number4
DOIs
StatePublished - Aug 15 2014

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Endothelial Cells
Sirolimus
Lysosomal acid lipase deficiency
Myeloid-Derived Suppressor Cells
Cell Proliferation
CD31 Antigens
Inflammation
Sterol Esterase
Deficiency Diseases
T-Lymphocytes
Blood Vessels
Reactive Oxygen Species
Leukocytes
Apoptosis

ASJC Scopus subject areas

  • Immunology

Cite this

Myeloid-derived suppressor cells are involved in lysosomal acid lipase deficiency-induced endothelial cell dysfunctions. / Zhao, Ting; Ding, Xinchun; Du, Hong; Yan, Cong.

In: Journal of Immunology, Vol. 193, No. 4, 15.08.2014, p. 1942-1953.

Research output: Contribution to journalArticle

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