MyoD and myogenin protein expression in skeletal muscles of senile rats

Eduard I. Dedkov, Tatiana Y. Kostrominova, Andrei B. Borisov, Bruce M. Carlson

Research output: Contribution to journalArticle

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Abstract

We analyzed the level of protein expression of two myogenic regulatory factors (MRFs), MyoD and myogenin, in senile skeletal muscles and determined the cellular source of their production in young adult (4 months old), old (24, 26, and 28 months old), and senile (32 months old) male rats. Immunoblotting demonstrated levels of myogenin ∼3.2, ∼4.0, and ∼5.5 times higher in gastrocnemius muscles of 24-, 26-, and 32-month-old animals, respectively, than in those of young adult rats. Anti-MyoD antibody recognized two major areas of immunoreactivity in Western blots: a single MyoD-specific band (∼43-45 kDa) and a double (or triple) MyoD-like band (∼55-65 kDa). Whereas the level of MyoD-specific protein in the 43- to 45-kDa band remained relatively unchanged during aging compared with that of young adult rats, the total level of MyoD-like immunoreactivity within the 55- to 65-kDa bands was ∼3.4, ∼4.7, ∼9.1, and ∼11.7 times higher in muscles of 24-, 26-, 28-, and 32-month-old rats, respectively. The pattern of MRF protein expression in intact senile muscles was similar to that recorded in young adult denervated muscles. Ultrastructural analysis of extensor digitorum longus muscle from senile rats showed that, occasionally, the area of the nerve-muscle junction was partially or completely devoid of axons, and satellite cells with the features of activated cells were found on the surface of living fibers. Immunohistochemistry detected accumulated MyoD and myogenin proteins in the nuclei of both fibers and satellite cells in 32-month-old muscles. We suggest that the up-regulated production of MyoD and myogenin proteins in the nuclei of both fibers and satellite cells could account for the high level of MRF expression in muscles of senile rats.

Original languageEnglish (US)
Pages (from-to)401-416
Number of pages16
JournalCell And Tissue Research
Volume311
Issue number3
StatePublished - Mar 1 2003

Fingerprint

MyoD Protein
Myogenin
Muscle
Rats
Skeletal Muscle
Myogenic Regulatory Factors
Muscles
Young Adult
Proteins
Satellites
Fibers
Immunoblotting
Axons
Anti-Idiotypic Antibodies
Western Blotting
Immunohistochemistry
Animals
Aging of materials

Keywords

  • Aging
  • Muscle fibers
  • MyoD
  • Myogenin
  • Rat (WI/HicksCar)
  • Satellite cells
  • Skeletal muscles

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

MyoD and myogenin protein expression in skeletal muscles of senile rats. / Dedkov, Eduard I.; Kostrominova, Tatiana Y.; Borisov, Andrei B.; Carlson, Bruce M.

In: Cell And Tissue Research, Vol. 311, No. 3, 01.03.2003, p. 401-416.

Research output: Contribution to journalArticle

Dedkov, EI, Kostrominova, TY, Borisov, AB & Carlson, BM 2003, 'MyoD and myogenin protein expression in skeletal muscles of senile rats', Cell And Tissue Research, vol. 311, no. 3, pp. 401-416.
Dedkov, Eduard I. ; Kostrominova, Tatiana Y. ; Borisov, Andrei B. ; Carlson, Bruce M. / MyoD and myogenin protein expression in skeletal muscles of senile rats. In: Cell And Tissue Research. 2003 ; Vol. 311, No. 3. pp. 401-416.
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T1 - MyoD and myogenin protein expression in skeletal muscles of senile rats

AU - Dedkov, Eduard I.

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AU - Borisov, Andrei B.

AU - Carlson, Bruce M.

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AB - We analyzed the level of protein expression of two myogenic regulatory factors (MRFs), MyoD and myogenin, in senile skeletal muscles and determined the cellular source of their production in young adult (4 months old), old (24, 26, and 28 months old), and senile (32 months old) male rats. Immunoblotting demonstrated levels of myogenin ∼3.2, ∼4.0, and ∼5.5 times higher in gastrocnemius muscles of 24-, 26-, and 32-month-old animals, respectively, than in those of young adult rats. Anti-MyoD antibody recognized two major areas of immunoreactivity in Western blots: a single MyoD-specific band (∼43-45 kDa) and a double (or triple) MyoD-like band (∼55-65 kDa). Whereas the level of MyoD-specific protein in the 43- to 45-kDa band remained relatively unchanged during aging compared with that of young adult rats, the total level of MyoD-like immunoreactivity within the 55- to 65-kDa bands was ∼3.4, ∼4.7, ∼9.1, and ∼11.7 times higher in muscles of 24-, 26-, 28-, and 32-month-old rats, respectively. The pattern of MRF protein expression in intact senile muscles was similar to that recorded in young adult denervated muscles. Ultrastructural analysis of extensor digitorum longus muscle from senile rats showed that, occasionally, the area of the nerve-muscle junction was partially or completely devoid of axons, and satellite cells with the features of activated cells were found on the surface of living fibers. Immunohistochemistry detected accumulated MyoD and myogenin proteins in the nuclei of both fibers and satellite cells in 32-month-old muscles. We suggest that the up-regulated production of MyoD and myogenin proteins in the nuclei of both fibers and satellite cells could account for the high level of MRF expression in muscles of senile rats.

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KW - Rat (WI/HicksCar)

KW - Satellite cells

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