Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function

Chandrashekara Mallappa, Brian T. Nasipak, Letitiah Etheridge, Elliot J. Androphy, Stephen N. Jones, Charles G. Sagerström, Yasuyuki Ohkawa, Anthony N. Imbalzano

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Knockdown of the Brg1 ATPase subunit of SWI/SNF chromatin remodeling enzymes in developing zebrafish caused stunted tail formation and altered sarcomeric actin organization, which phenocopies the loss of the microRNA processing enzyme Dicer, or the knockdown of myogenic microRNAs. Furthermore, myogenic microRNA expression and differentiation was blocked in Brg1 conditional myoblasts differentiated ex vivo. The binding of Brg1 upstream of myogenic microRNA sequences correlated with MyoD binding and accessible chromatin structure in satellite cells and myofibers, and it was required for chromatin accessibility and microRNA expression in a tissue culture model for myogenesis. The results implicate ATP-dependent chromatin remodelers in myogenic microRNA gene regulation.

Original languageEnglish (US)
Pages (from-to)3176-3186
Number of pages11
JournalMolecular and cellular biology
Volume30
Issue number13
DOIs
StatePublished - Jul 1 2010
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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  • Cite this

    Mallappa, C., Nasipak, B. T., Etheridge, L., Androphy, E. J., Jones, S. N., Sagerström, C. G., Ohkawa, Y., & Imbalzano, A. N. (2010). Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function. Molecular and cellular biology, 30(13), 3176-3186. https://doi.org/10.1128/MCB.00214-10