Myosin II Light Chain Phosphorylation Regulates Membrane Localization and Apoptotic Signaling of Tumor Necrosis Factor Receptor-1

Yijun Jin, Simon J. Atkinson, James A. Marrs, Patricia Gallagher

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Activation of myosin II by myosin light chain kinase (MLCK) produces the force for many cellular processes including muscle contraction, mitosis, migration, and other cellular shape changes. The results of this study show that inhibition or potentiation of myosin II activation via over-expression of a dominant negative or wild type MLCK can delay or accelerate tumor necrosis factor-α (TNF)-induced apoptotic cell death in cells. Changes in the activation of caspase-8 that parallel changes in regulatory light chain phosphorylation levels reveal that myosin II motor activities regulate TNF receptor-1 (TNFR-1) signaling at an early step in the TNF death signaling pathway. Treatment of cells with either ionomycin or endotoxin (lipopolysaccharide) leads to activation of myosin II and increased translocation of TNFR-1 to the plasma membrane independent of TNF signaling. The results of these studies establish a new role for myosin II motor activity in regulating TNFR-1-mediated apoptosis through the translocation of TNFR-1 to or within the plasma membrane.

Original languageEnglish
Pages (from-to)30342-30349
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number32
DOIs
StatePublished - Aug 10 2001

Fingerprint

Myosin Type II
Myosin Light Chains
Phosphorylation
Tumor Necrosis Factor Receptors
Membranes
Chemical activation
Myosin-Light-Chain Kinase
Tumor Necrosis Factor-alpha
Cell membranes
Motor Activity
Cell Membrane
Ionomycin
Caspase 8
Cell death
Muscle Contraction
Mitosis
Endotoxins
Lipopolysaccharides
Muscle
Cell Death

ASJC Scopus subject areas

  • Biochemistry

Cite this

Myosin II Light Chain Phosphorylation Regulates Membrane Localization and Apoptotic Signaling of Tumor Necrosis Factor Receptor-1. / Jin, Yijun; Atkinson, Simon J.; Marrs, James A.; Gallagher, Patricia.

In: Journal of Biological Chemistry, Vol. 276, No. 32, 10.08.2001, p. 30342-30349.

Research output: Contribution to journalArticle

Jin, Yijun ; Atkinson, Simon J. ; Marrs, James A. ; Gallagher, Patricia. / Myosin II Light Chain Phosphorylation Regulates Membrane Localization and Apoptotic Signaling of Tumor Necrosis Factor Receptor-1. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 32. pp. 30342-30349.
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