N-3 Fatty acids prevent whereas trans-fatty acids induce vascular inflammation and sudden cardiac death

Rafat A. Siddiqui, Kevin A. Harvey, Nargiz Ruzmetov, Steven Miller, Gary P. Zaloga

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

n-3 PUFA have well-recognised cardio-beneficial effects. In contrast, premature coronary deaths are associated with consumption of high levels of trans-fatty acids (TFA). The present study determined the effects of n-3 PUFA and TFA on sudden cardiac death and vascular inflammation. A rat coronary ligation model was used to study the effect of fatty acids on sudden cardiac death, whereas a mouse femoral artery ligation model was used to study compensatory vascular remodelling. Human aortic endothelial cells (HAEC) were utilised for the in vitro studies to investigate expression of inflammatory molecules. Feeding animals an n-3 PUFA-enriched diet caused a sevenfold increase in plasma n-3 PUFA compared with that of the TFA-fed group, whereas a TFA-enriched diet caused a 25-fold increase in plasma TFA compared with the n-3 PUFA group. Animals on a TFA diet had a lower survival rate due to sudden cardiac death and exhibited variable degrees of aortic atherosclerotic lesions. Animals on a TFA diet had diminished hindlimb collateral growth, whereas animals on the n-3 PUFA diet exhibited extensive collateral growth about ligated regions. HAEC treated with TFA (trans-18:2) showed significantly increased expression of intracellular adhesion molecule-1 and nitrosylation of cellular proteins than those treated with DHA (n-3 PUFA, 22:6). The in vivo study demonstrates that, in contrast to TFA, n-3 PUFA improve animal survival after myocardial infarction, prevent development of atherosclerotic lesions and stimulate compensatory vascular remodelling. The in vitro study demonstrates that TFA induce, while n-3 PUFA prevent, vascular inflammation.

Original languageEnglish
Pages (from-to)1811-1819
Number of pages9
JournalBritish Journal of Nutrition
Volume102
Issue number12
DOIs
StatePublished - Dec 2009

Fingerprint

Trans Fatty Acids
Sudden Cardiac Death
Omega-3 Fatty Acids
Blood Vessels
Inflammation
Diet
Ligation
Endothelial Cells
Premature Mortality
Femoral Artery
Hindlimb
Growth
Fatty Acids
Survival Rate
Myocardial Infarction

Keywords

  • Inflammation
  • N-3 Fatty acids
  • Sudden cardiac death
  • Trans-Fatty acids
  • Vascular remodelling

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Medicine(all)

Cite this

N-3 Fatty acids prevent whereas trans-fatty acids induce vascular inflammation and sudden cardiac death. / Siddiqui, Rafat A.; Harvey, Kevin A.; Ruzmetov, Nargiz; Miller, Steven; Zaloga, Gary P.

In: British Journal of Nutrition, Vol. 102, No. 12, 12.2009, p. 1811-1819.

Research output: Contribution to journalArticle

Siddiqui, Rafat A. ; Harvey, Kevin A. ; Ruzmetov, Nargiz ; Miller, Steven ; Zaloga, Gary P. / N-3 Fatty acids prevent whereas trans-fatty acids induce vascular inflammation and sudden cardiac death. In: British Journal of Nutrition. 2009 ; Vol. 102, No. 12. pp. 1811-1819.
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