N-acetyl-beta-D-glucosaminidase (NAG) expression by LLC-PK1 and IMCD kidney cells following exposure to pathological conditions in vitro

Karen Haberstroh, Martin Kaefer, Rena Bizios

Research output: Contribution to journalArticle

Abstract

Kidney tubular epithelial cells (LLC-PK1) and inner medullary collecting duct cells (mlMCD-3) were exposed to either 60 cm H20 pressure, or to a 95%N2/5%CO2 environment under 0.3 or 60 cm H2O pressure, under standard cell culture conditions for 5 consecutive hours. Expression of N-Acetyl-Beta-D-glucosaminidase (NAG) by LLC-PK1 cells was not increased under any conditions. In contrast, increased IMCD NAG levels provided the first cellular-level evidence that kidney cell damage in the inner medullary region may be related to both renal hypoxia and exposure to elevated hydrostatic pressure.

Original languageEnglish (US)
JournalAnnals of Biomedical Engineering
Volume28
Issue numberSUPPL. 1
StatePublished - 2000
Externally publishedYes

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Hydrostatic pressure
Cell culture
Ducts
Cells
Epithelial Cells

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

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abstract = "Kidney tubular epithelial cells (LLC-PK1) and inner medullary collecting duct cells (mlMCD-3) were exposed to either 60 cm H20 pressure, or to a 95{\%}N2/5{\%}CO2 environment under 0.3 or 60 cm H2O pressure, under standard cell culture conditions for 5 consecutive hours. Expression of N-Acetyl-Beta-D-glucosaminidase (NAG) by LLC-PK1 cells was not increased under any conditions. In contrast, increased IMCD NAG levels provided the first cellular-level evidence that kidney cell damage in the inner medullary region may be related to both renal hypoxia and exposure to elevated hydrostatic pressure.",
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T1 - N-acetyl-beta-D-glucosaminidase (NAG) expression by LLC-PK1 and IMCD kidney cells following exposure to pathological conditions in vitro

AU - Haberstroh, Karen

AU - Kaefer, Martin

AU - Bizios, Rena

PY - 2000

Y1 - 2000

N2 - Kidney tubular epithelial cells (LLC-PK1) and inner medullary collecting duct cells (mlMCD-3) were exposed to either 60 cm H20 pressure, or to a 95%N2/5%CO2 environment under 0.3 or 60 cm H2O pressure, under standard cell culture conditions for 5 consecutive hours. Expression of N-Acetyl-Beta-D-glucosaminidase (NAG) by LLC-PK1 cells was not increased under any conditions. In contrast, increased IMCD NAG levels provided the first cellular-level evidence that kidney cell damage in the inner medullary region may be related to both renal hypoxia and exposure to elevated hydrostatic pressure.

AB - Kidney tubular epithelial cells (LLC-PK1) and inner medullary collecting duct cells (mlMCD-3) were exposed to either 60 cm H20 pressure, or to a 95%N2/5%CO2 environment under 0.3 or 60 cm H2O pressure, under standard cell culture conditions for 5 consecutive hours. Expression of N-Acetyl-Beta-D-glucosaminidase (NAG) by LLC-PK1 cells was not increased under any conditions. In contrast, increased IMCD NAG levels provided the first cellular-level evidence that kidney cell damage in the inner medullary region may be related to both renal hypoxia and exposure to elevated hydrostatic pressure.

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JO - Annals of Biomedical Engineering

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