N-acetyl-beta-D-glucosaminidase (NAG) expression by LLC-PK1 and IMCD kidney cells following exposure to pathological conditions in vitro

Karen Haberstroh, Martin Kaefer, Rena Bizios

Research output: Contribution to journalConference article

Abstract

Kidney tubular epithelial cells (LLC-PK1) and inner medullary collecting duct cells (mlMCD-3) were exposed to either 60 cm H20 pressure, or to a 95%N2/5%CO2 environment under 0.3 or 60 cm H2O pressure, under standard cell culture conditions for 5 consecutive hours. Expression of N-Acetyl-Beta-D-glucosaminidase (NAG) by LLC-PK1 cells was not increased under any conditions. In contrast, increased IMCD NAG levels provided the first cellular-level evidence that kidney cell damage in the inner medullary region may be related to both renal hypoxia and exposure to elevated hydrostatic pressure.

Original languageEnglish (US)
Pages (from-to)S-50
JournalAnnals of Biomedical Engineering
Volume28
Issue numberSUPPL. 1
StatePublished - Dec 1 2000
Externally publishedYes
Event2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA
Duration: Oct 12 2000Oct 14 2000

ASJC Scopus subject areas

  • Biomedical Engineering

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