N- and P/Q-type Ca2+ channels in adrenal chromaffin cells

A. P. Fox, A. L. Cahill, K. P M Currie, C. Grabner, A. B. Harkins, B. Herring, Joyce Hurley, Z. Xie

Research output: Contribution to journalArticle

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Abstract

Ca2+ is the most ubiquitous second messenger found in all cells. Alterations in [Ca2+]i contribute to a wide variety of cellular responses including neurotransmitter release, muscle contraction, synaptogenesis and gene expression. Voltage-dependent Ca2+ channels, found in all excitable cells (Hille 1992), mediate the entry of Ca2+ into cells following depolarization. Ca2+ channels are composed of a large pore-forming subunit, called the α1 subunit, and several accessory subunits. Ten different α1 subunit genes have been identified and classified into three families, Cav1-3 (Dunlap et al. 1995, Catterall 2000). Each α1 gene produces a unique Ca 2+ channel. Although chromaffin cells express several different types of Ca2+ channels, this review will focus on the Cav2.1 and Cav2.2 channels, also known as P/Q- and N-type respectively (Nowycky et al. 1985, Llinas et al. 1989b, Wheeler et al. 1994). These channels exhibit physiological and pharmacological properties similar to their neuronal counterparts. N-, P/Q and to a lesser extent R-type Ca2+ channels are known to regulate neurotransmitter release (Hirning et al. 1988, Horne & Kemp 1991, Uchitel et al. 1992, Luebke et al. 1993, Takahashi & Momiyama 1993, Turner et al. 1993, Regehr & Mintz 1994, Wheeler et al. 1994, Wu & Saggau 1994, Waterman 1996, Wright & Angus 1996, Reid et al. 1997). N- and P/Q-type Ca2+ channels are abundant in nerve terminals where they colocalize with synaptic vesicles. Similarly, these channels play a role in neurotransmitter release in chromaffin cells (Garcia et al. 2006). N- and P/Q-type channels are subject to many forms of regulation (Ikeda & Dunlap 1999). This review pays particular attention to the regulation of N- and P/Q-type channels by heterotrimeric G-proteins, interaction with SNARE proteins, and channel inactivation in the context of stimulus-secretion coupling in adrenal chromaffin cells.

Original languageEnglish
Pages (from-to)247-261
Number of pages15
JournalActa Physiologica
Volume192
Issue number2
DOIs
StatePublished - Feb 2008

Fingerprint

Chromaffin Cells
Neurotransmitter Agents
SNARE Proteins
Heterotrimeric GTP-Binding Proteins
Synaptic Vesicles
Second Messenger Systems
Muscle Contraction
Genes
Pharmacology
Gene Expression

Keywords

  • Ca channels
  • Inactivation
  • Palmitoylation
  • SNAREs
  • Stimulus-secretion coupling
  • Voltage-dependent inhibition

ASJC Scopus subject areas

  • Physiology

Cite this

Fox, A. P., Cahill, A. L., Currie, K. P. M., Grabner, C., Harkins, A. B., Herring, B., ... Xie, Z. (2008). N- and P/Q-type Ca2+ channels in adrenal chromaffin cells. Acta Physiologica, 192(2), 247-261. https://doi.org/10.1111/j.1748-1716.2007.01817.x

N- and P/Q-type Ca2+ channels in adrenal chromaffin cells. / Fox, A. P.; Cahill, A. L.; Currie, K. P M; Grabner, C.; Harkins, A. B.; Herring, B.; Hurley, Joyce; Xie, Z.

In: Acta Physiologica, Vol. 192, No. 2, 02.2008, p. 247-261.

Research output: Contribution to journalArticle

Fox, AP, Cahill, AL, Currie, KPM, Grabner, C, Harkins, AB, Herring, B, Hurley, J & Xie, Z 2008, 'N- and P/Q-type Ca2+ channels in adrenal chromaffin cells', Acta Physiologica, vol. 192, no. 2, pp. 247-261. https://doi.org/10.1111/j.1748-1716.2007.01817.x
Fox AP, Cahill AL, Currie KPM, Grabner C, Harkins AB, Herring B et al. N- and P/Q-type Ca2+ channels in adrenal chromaffin cells. Acta Physiologica. 2008 Feb;192(2):247-261. https://doi.org/10.1111/j.1748-1716.2007.01817.x
Fox, A. P. ; Cahill, A. L. ; Currie, K. P M ; Grabner, C. ; Harkins, A. B. ; Herring, B. ; Hurley, Joyce ; Xie, Z. / N- and P/Q-type Ca2+ channels in adrenal chromaffin cells. In: Acta Physiologica. 2008 ; Vol. 192, No. 2. pp. 247-261.
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