N-myristoylation is required for function of the pheromone-responsive G(α) protein of yeast: Conditional activation of the pheromone response by a temperature-sensitive N-myristoyl transferase

D. E. Stone, G. M. Cole, M. De Barros Lopes, M. Goebl, S. I. Reed

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

In a screen designed to identify novel mutations in the mating response pathway of Saccharomyces cerevisiae, we isolated conditional alleles of NMT1, the gene encoding N-myristoyl transferase. Genetic data indicate that Nmt1 deficiency results in the activation of the pheromone response at the level of Gpa1, the α subunit of the pheromone-responsive G protein. We show that Gpa1 is myristoylated by Nmt1, and without this normally stable modification, Gpa1 is unable to inhibit pheromone signaling. This loss of Gpa1 function is probably not the result of improper subcellular localization. Unlike the mammalian Gαi proteins αi and αo, nonmyristoylated Gpa1 is able to associate with membranes. In addition to Gpa1, our data indicate that Nmt1 myristoylates other proteins essential to vegetative growth.

Original languageEnglish (US)
Pages (from-to)1969-1981
Number of pages13
JournalGenes and Development
Volume5
Issue number11
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Keywords

  • Amino-terminal myristoylation
  • G protein
  • Gpa1
  • N-myristoyl transferase
  • Pheromone response
  • Yeast

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

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