Nanometer resolution hard X-ray microscopy of bone and mineralized tissue

M. E. Ruppel, Y. Meng, Y. Qin, D. B. Burr, M. R. Allen, Y. F. Song, L. M. Miller

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Full field hard x-ray microscopy has recently been developed utilizing synchrotron light, enabling absorption and phase contrast imaging with a spatial resolution of 50 - 100 nm. This technique has allowed us to image bone tissue at the nanoscale level, viewing the substructure of mineralized collagen fibers. Thin sections of tissue (5 and 50 μm thick) from the L3 vertebrae of Beagles and mineralizing MC3T3-E1 mouse osteoblasts were imaged at beamline 01B1 at the National Synchrotron Radiation Research Center (Hsinchu, Taiwan). Collagen fiber orientation was observed in the lamellar structure of an osteon. Mineralizing osteoblasts were found to produce disordered mineralized nodules with varying density and structure.

Original languageEnglish (US)
Title of host publication33rd Annual Northeast Bioengineering Conference - Engineering Innovations in Life Sciences and Healthcare, NEBC
Pages33-34
Number of pages2
DOIs
StatePublished - Dec 1 2007
Event33rd Annual Northeast Bioengineering Conference, NEBC - Stony Brook, NY, United States
Duration: Mar 10 2007Mar 11 2007

Publication series

NameBioengineering, Proceedings of the Northeast Conference
ISSN (Print)1071-121X

Other

Other33rd Annual Northeast Bioengineering Conference, NEBC
CountryUnited States
CityStony Brook, NY
Period3/10/073/11/07

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Ruppel, M. E., Meng, Y., Qin, Y., Burr, D. B., Allen, M. R., Song, Y. F., & Miller, L. M. (2007). Nanometer resolution hard X-ray microscopy of bone and mineralized tissue. In 33rd Annual Northeast Bioengineering Conference - Engineering Innovations in Life Sciences and Healthcare, NEBC (pp. 33-34). [4413266] (Bioengineering, Proceedings of the Northeast Conference). https://doi.org/10.1109/NEBC.2007.4413266