Na+-driven multidrug efflux pump VcmA from Vibrio cholerae non-O1, a non-halophilic bacterium

M. Nazmul Huda, Yuji Morita, Teruo Kuroda, Tohru Mizushima, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

A chromosomal DNA fragment from Vibrio cholerae non-O1 containing a drug resistance determinant was cloned and sequenced. The deduced amino acid sequence suggested that the determinant gene encodes a multidrug efflux pump. We designated the pump VcmA. Escherichia coli cells transformed with a plasmid carrying the vcmA gene showed increased resistance against norfloxacin, ciprofloxacin, ofloxacin, daunomycin, doxorubicin, streptomycin, kanamycin, ethidium bromide, 4′,6-diamidino-2-phenylindole dihydrochloride, Hoechst 33342 and acriflavine. Na+ (or Li+)-dependent efflux of ethidium bromide was detected in transformant cells. Efflux of Na+, elicited by ethidium bromide, was observed from transformant cells. Thus, we concluded that the VcmA is a Na+/drug antiporter.

Original languageEnglish (US)
Pages (from-to)235-239
Number of pages5
JournalFEMS Microbiology Letters
Volume203
Issue number2
DOIs
StatePublished - Sep 25 2001
Externally publishedYes

Keywords

  • Drug resistance
  • Na/drug antiporter
  • VcmA drug efflux pump
  • Vibrio cholerae

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Na<sup>+</sup>-driven multidrug efflux pump VcmA from Vibrio cholerae non-O1, a non-halophilic bacterium'. Together they form a unique fingerprint.

  • Cite this