Native cytosolic protein phosphatase-1 (PP-1S) containing modulator (inhibitor-2) is an active enzyme

Mathieu Bollen, Anna De Paoli-Roach, Willy Stalmans

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In vitro, the modulator protein (inhibitor-2) slowly converts the catalytic subunit of protein phosphatase-1 (PP-1c) into an inactive 'MgATP-dependent form' that can be reactivated by the transient phosphorylation of modulator with GSK-3/FA. We report here that this modulator-induced inactivation of PP-1C can be blocked by addition (at pH 7.5) of either 0.3 mM NaF or 150 mM NaCl, or by raising the pH to 8.5. Making use of a combination of the latter conditions, we have partially purified a soluble modulator-associated form of PP-1 (PP-1S) from rabbit skeletal muscle as a spontaneously active enzyme that cannot be further activated by kinase GSK-3/FA. These observations argue against a role for the 'MgATP-dependent' form of PP-1S as an inactive reservoir of PP-1C. PP-1S was separated on aminohexyl Sepharose from another active, cytosolic species of PP-1, which appears to be a proteolytic product of the glycogen-bound PP-1G.

Original languageEnglish
Pages (from-to)196-200
Number of pages5
JournalFEBS Letters
Volume344
Issue number2-3
DOIs
StatePublished - May 16 1994

Fingerprint

Glycogen Synthase Kinase 3
Protein Phosphatase 1
Modulators
Adenosine Triphosphate
Enzymes
Glycogen
Skeletal Muscle
Phosphotransferases
Phosphorylation
Rabbits
Muscle
Proteins
In Vitro Techniques
aminohexyl-sepharose

Keywords

  • Chaperone
  • Inhibitor-2
  • Protein phosphatase
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Native cytosolic protein phosphatase-1 (PP-1S) containing modulator (inhibitor-2) is an active enzyme. / Bollen, Mathieu; De Paoli-Roach, Anna; Stalmans, Willy.

In: FEBS Letters, Vol. 344, No. 2-3, 16.05.1994, p. 196-200.

Research output: Contribution to journalArticle

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