Natural killer cells do not mediate facial motoneuron survival after facial nerve transection

Susanna C. Byram, Craig J. Serpe, Stephen B. Pruett, Virginia M. Sanders, Kathryn Jones

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The goal of the current study was to determine if natural killer (NK) cells mediate facial motoneuron (FMN) survival following injury. Wild-type (WT), perforin/recombinase activating gene-2 knockout (pfp/RAG-2 KO), and common γ-chain (γc)/RAG-2 KO mice received a right facial nerve axotomy. In WT mice, FMN survival was 86±1.0% relative to the contralateral control side. In contrast, pfp/RAG-2 and γc/RAG-2 KO mice exhibited significant decreases in FMN survival (∼20% and ∼30%, respectively), relative to WT. Reconstitution of pfp/RAG-2 and γc/RAG-2 KO mice with normal NK cells alone, failed to restore FMN survival levels to those of WT, but did restore functional lytic activity against YAC-1 cells. Reconstitution of pfp/RAG-2 and γc/RAG-2 KO mice with splenocytes, and pfp/RAG-2 KO mice with CD4+ T-lymphocytes alone or in combination with NK cells, restored FMN survival levels to those of WT. Thus, NK cells appear to not be a component of immune cell-mediated rescue of motoneurons from axotomy induced cell death.

Original languageEnglish (US)
Pages (from-to)417-425
Number of pages9
JournalBrain, Behavior, and Immunity
Volume17
Issue number6
DOIs
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

Facial Nerve
Motor Neurons
Natural Killer Cells
Axotomy
Perforin
Gene Knockout Techniques
Recombinases
Cellular Structures
Cell Death
T-Lymphocytes
Wounds and Injuries

Keywords

  • Facial nerve
  • FMN
  • Innate immunity
  • Natural killer cells
  • Neuro-immune interactions
  • Neuroimmunology
  • Neuronal survival
  • NK cells
  • Peripheral nerve injury
  • Reconstitution

ASJC Scopus subject areas

  • Immunology
  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems

Cite this

Natural killer cells do not mediate facial motoneuron survival after facial nerve transection. / Byram, Susanna C.; Serpe, Craig J.; Pruett, Stephen B.; Sanders, Virginia M.; Jones, Kathryn.

In: Brain, Behavior, and Immunity, Vol. 17, No. 6, 12.2003, p. 417-425.

Research output: Contribution to journalArticle

Byram, Susanna C. ; Serpe, Craig J. ; Pruett, Stephen B. ; Sanders, Virginia M. ; Jones, Kathryn. / Natural killer cells do not mediate facial motoneuron survival after facial nerve transection. In: Brain, Behavior, and Immunity. 2003 ; Vol. 17, No. 6. pp. 417-425.
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