Neonatal stem cells exhibit specific characteristics in function, proliferation, and cellular signaling that distinguish them from their adult counterparts

Troy A. Markel, Meijing Wang, Paul R. Crisostomo, Maiuxi C. Manukyan, Jeffrey A. Poynter, Daniel R. Meldrum

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Stem cells may be a novel treatment modality for organ ischemia, possibly through beneficial paracrine mechanisms. Stem cells from older hosts have been shown to exhibit decreased function during stress. We therefore hypothesized that 1) neonatal bone marrow mesenchymal stem cells (nBMSCs) would produce different levels of IL-6, VEGF, and IGF-1 compared with adults (aBMSCs) when stimulated with TNF or LPS; 2) differences in cytokines would be due to distinct cellular characteristics, such as proliferation or pluripotent potential; and 3) differences in cytokines would be associated with differences in p38 MAPK and ERK signaling within nBMSCs. BMSCs were isolated from adult and neonatal mice. Cells were exposed to TNF or LPS with or without p38 or ERK inhibition. Growth factors were measured via ELISA, proliferation via daily cell counts, cell surface markers via flow cytometry, and pluripotent potential via alkaline phosphatase activity. nBMSCs produced lower levels of IL-6 and VEGF, but higher levels of IGF-1 under basal conditions, as well as after stimulation with TNF, but not LPS. Neonatal and adult BMSCs had similar pluripotent potentials and cell surface markers, but nBMSCs proliferated faster. Furthermore, p38 and ERK appeared to play a more substantial role in nBMSC cytokine and growth factor production. Neonatal stem cells may aid in decreasing the local inflammatory response during ischemia, and could possibly be expanded more rapidly than adult cells prior to therapeutic use.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume294
Issue number5
DOIs
StatePublished - May 2008

Fingerprint

Mesenchymal Stromal Cells
Stem Cells
Bone Marrow
Cell Proliferation
Cytokines
Insulin-Like Growth Factor I
Vascular Endothelial Growth Factor A
Interleukin-6
Intercellular Signaling Peptides and Proteins
Ischemia
Stem Cell Factor
Therapeutic Uses
p38 Mitogen-Activated Protein Kinases
Alkaline Phosphatase
Flow Cytometry
Cell Count
Enzyme-Linked Immunosorbent Assay
Therapeutics

Keywords

  • Fetal
  • Insulin-like growth factor-1
  • Interleukin-6
  • Ischemia
  • Stem cell therapy
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Neonatal stem cells exhibit specific characteristics in function, proliferation, and cellular signaling that distinguish them from their adult counterparts. / Markel, Troy A.; Wang, Meijing; Crisostomo, Paul R.; Manukyan, Maiuxi C.; Poynter, Jeffrey A.; Meldrum, Daniel R.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 294, No. 5, 05.2008.

Research output: Contribution to journalArticle

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