Neurine, an acetylcholine autolysis product, elevates secreted amyloid-β protein precursor and amyloid-β peptide levels, and lowers neuronal cell viability in culture

A role in Alzheimer's disease?

David Tweedie, Arnold Brossi, DeMoa Chen, Yuan Wen Ge, Jason Bailey, Qian Sheng Yu, Mohammad A. Kamal, Kumar Sambamurti, Debomoy Lahiri, Nigel H. Greig

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Classical hallmarks of Alzheimer's disease (AD) are a synaptic loss, cholinergic neuron death, and abnormal protein deposition, particularly of toxic amyloid-β peptide (Aβ) that is derived from amyloid-β protein precursor (AβPP) by the action of beta- and gamma-secretases. The trigger(s) initiating the biochemical cascades that underpin these hallmarks have yet to be fully elucidated. The typical forebrain cholinergic cell demise associated with AD brain results in a loss of presynaptic cholinergic markers and acetylcholine (ACh). Neurine (vinyl-trimethyl-ammonium hydroxide) is a breakdown product of ACh, consequent to autolysis and is an organic poison found in cadavre brain. The time- and concentration-dependent actions of neurine were assessed in human neuroblastoma (NB, SK-N-SH) cells in culture by quantifying cell viability by lactate dehydrogenase (LDH) and MTS assay, and AβPP and Aβ levels by Western blot and ELISA. NB cells displayed evidence of toxicity to neurine at ≥3 mg/ml, as demonstrated by elevated LDH levels in the culture media and a reduced cell viability shown by the MTS assay. Using subtoxic concentrations of neurine, elevations in AβPP and Aβ 1-40 peptide levels were detected in conditioned media samples.

Original languageEnglish
Pages (from-to)9-16
Number of pages8
JournalJournal of Alzheimer's Disease
Volume10
Issue number1
StatePublished - 2006

Fingerprint

Autolysis
Amyloid beta-Protein Precursor
Amyloid
Acetylcholine
Cell Survival
Amyloid Precursor Protein Secretases
Alzheimer Disease
Poisons
Staphylococcal Protein A
L-Lactate Dehydrogenase
Cholinergic Agents
Peptides
Ammonium Hydroxide
Cholinergic Neurons
Brain
Conditioned Culture Medium
Prosencephalon
Neuroblastoma
Culture Media
Cell Culture Techniques

Keywords

  • Acetylcholine
  • Alzheimer's disease
  • Amyloid-β peptide
  • Amyloid-β protein precursor
  • Neurine
  • Ptomaine

ASJC Scopus subject areas

  • Neuropsychology and Physiological Psychology

Cite this

Neurine, an acetylcholine autolysis product, elevates secreted amyloid-β protein precursor and amyloid-β peptide levels, and lowers neuronal cell viability in culture : A role in Alzheimer's disease? / Tweedie, David; Brossi, Arnold; Chen, DeMoa; Ge, Yuan Wen; Bailey, Jason; Yu, Qian Sheng; Kamal, Mohammad A.; Sambamurti, Kumar; Lahiri, Debomoy; Greig, Nigel H.

In: Journal of Alzheimer's Disease, Vol. 10, No. 1, 2006, p. 9-16.

Research output: Contribution to journalArticle

Tweedie, David ; Brossi, Arnold ; Chen, DeMoa ; Ge, Yuan Wen ; Bailey, Jason ; Yu, Qian Sheng ; Kamal, Mohammad A. ; Sambamurti, Kumar ; Lahiri, Debomoy ; Greig, Nigel H. / Neurine, an acetylcholine autolysis product, elevates secreted amyloid-β protein precursor and amyloid-β peptide levels, and lowers neuronal cell viability in culture : A role in Alzheimer's disease?. In: Journal of Alzheimer's Disease. 2006 ; Vol. 10, No. 1. pp. 9-16.
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