Neuronal adrenergic and muscular cholinergic contractile hypersensitivity in canine jejunum after extrinsic denervation

Bruno M. Balsiger, Chong Liang He, Nicholas Zyromski, Michael G. Sarr

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Extrinsic denervation may be responsible for motor dysfunction after small bowel transplantation. The aim of this study was to examine the role of extrinsic innervation of canine jejunum on contractile activity. An in vitro dose response of cholinergic and adrenergic agonists was evaluated in canine jejunal strips of circular muscle at 0, 2, and 8 weeks in a control group and after jejunoileal extrinsic denervation (EX DEN). Neurons in circular muscle were quantitated by means of immunohistochemical techniques. Adrenergic and cholinergic responses did not differ at any time in the control group. However, at 2 and 8 weeks, extrinsic denervation caused an increased sensitivity to the procontractile effects of the cholinergic agonist bethanechol at the level of the smooth muscle cells, and increased sensitivity to the inhibitory effects of the adrenergic agent norepinephrine mediated at the level of the enteric nervous system. Immunohistochemical analysis showed a reduction in all neurons and a complete lack of adrenergic fibers in the EX DEN group after 2 and 8 weeks. Extrinsic denervation induces enteric neuronal cholinergic and adrenergic smooth muscle hypersensitivity in canine jejunal circular muscle.

Original languageEnglish (US)
Pages (from-to)572-582
Number of pages11
JournalJournal of Gastrointestinal Surgery
Volume7
Issue number4
DOIs
StatePublished - May 2003
Externally publishedYes

Fingerprint

Denervation
Jejunum
Adrenergic Agents
Cholinergic Agents
Canidae
Hypersensitivity
Cholinergic Agonists
Muscles
Bethanechol
Enteric Nervous System
Adrenergic Fibers
Neurons
Adrenergic Agonists
Control Groups
Smooth Muscle Myocytes
Smooth Muscle
Norepinephrine
Transplantation

Keywords

  • Adrenergic nerves
  • Cholinergic nerves
  • Contractility
  • Denervation
  • Denervation hypersensitivity
  • Motility
  • Small intestine
  • Smooth muscle contractions

ASJC Scopus subject areas

  • Surgery

Cite this

Neuronal adrenergic and muscular cholinergic contractile hypersensitivity in canine jejunum after extrinsic denervation. / Balsiger, Bruno M.; He, Chong Liang; Zyromski, Nicholas; Sarr, Michael G.

In: Journal of Gastrointestinal Surgery, Vol. 7, No. 4, 05.2003, p. 572-582.

Research output: Contribution to journalArticle

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