Neuronal subtype-specific genes that control corticospinal motor neuron development in vivo

Paola Arlotta, Bradley J. Molyneaux, Jinhui Chen, Jun Inoue, Ryo Kominami, Jeffrey D. MacKlis

Research output: Contribution to journalArticle

672 Scopus citations

Abstract

Within the vertebrate nervous system, the presence of many different lineages of neurons and glia complicates the molecular characterization of single neuronal populations. In order to elucidate molecular mechanisms underlying the specification and development of corticospinal motor neurons (CSMN), we purified CSMN at distinct stages of development in vivo and compared their gene expression to two other pure populations of cortical projection neurons: callosal projection neurons and corticotectal projection neurons. We found genes that are potentially instructive for CSMN development, as well as genes that are excluded from CSMN and are restricted to other populations of neurons, even within the same cortical layer. Loss-of-function experiments in null mutant mice for Ctip2 (also known as Bcl11b), one of the newly characterized genes, demonstrate that it plays a critical role in the development of CSMN axonal projections to the spinal cord in vivo, confirming that we identified central genetic determinants of the CSMN population.

Original languageEnglish (US)
Pages (from-to)207-221
Number of pages15
JournalNeuron
Volume45
Issue number2
DOIs
StatePublished - Jan 20 2005
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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    Arlotta, P., Molyneaux, B. J., Chen, J., Inoue, J., Kominami, R., & MacKlis, J. D. (2005). Neuronal subtype-specific genes that control corticospinal motor neuron development in vivo. Neuron, 45(2), 207-221. https://doi.org/10.1016/j.neuron.2004.12.036