Neuropeptide organization of the hypothalamic projection to the parabrachial nucleus in the rat

Margaret Moga, C. B. Saper, T. S. Gray

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

The hypothalamus is a major source of afferents to the parabrachial nucleus (PB), but the neurotransmitters in this pathway are largely unknown. In this study, we examine the neuropeptide immunoreactivities of neurons in the hypothalamus that project to the PB by using the combined retrograde fluorescence-immunofluorescence method. After injections of the fluorescent tracer blue into the PB, retrogradely labeled neurons were oberved in the paraventricular, dorsomedial, ventromedial, median preoptic, and anteroventral periventricular hypothalamic nuclei; in the dorsal, retrochiasmatic, and lateral hypothalamic areas; and in the medial and lateral preoptic areas. Our results show that at least five distinct neuropeptide-immunoreactive cell populations in the hypothalamus project to the PB. In the perifornical lateral hypothalamus, many neurotensin (NT)-, corticotropin-releasing factor-, dynorphin (DYN)-, angiotensin II (AII)-, and galanin-like immunoreactive (-ir) neurons were retrogradely labeled. A cluster of retrogradely labeled neurons in the juxtacapsular lateral hypothalamus stained with an antiserum against α-melanocyte stimulating hormone (αMSH). Over 50% of the retrogradely labeled cells in the arcuate nucleus were adrenocorticotropin (ACTH)-or αMSH-ir. Many αMSH- and ACTH-ir, and a few DYN-, NT- and AII-ir neurons in the retrochiasmatic area were retrogradely labeled. Only small numbers of double-labeled neurons were found in the paraventricular nucleus, and, of these, enkephalin-ir and dyhorphin-ir neurons were the most common. Somatostatin-ir cells in the hypothalamus were rarely double-labeled. The chemical coding of these hypothalamic projections to the PB may provide important clues to the functional organization of these descending pathways.

Original languageEnglish (US)
Pages (from-to)662-682
Number of pages21
JournalJournal of Comparative Neurology
Volume295
Issue number4
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Neuropeptides
Neurons
Lateral Hypothalamic Area
Melanocyte-Stimulating Hormones
Hypothalamus
Adrenocorticotropic Hormone
Dynorphins
Neurotensin
Preoptic Area
Angiotensin II
Anterior Hypothalamus
Galanin
Arcuate Nucleus of Hypothalamus
Somatostatin-Secreting Cells
Paraventricular Hypothalamic Nucleus
Enkephalins
Corticotropin-Releasing Hormone
Parabrachial Nucleus
Fluorescent Antibody Technique
Neurotransmitter Agents

Keywords

  • angiotensin II
  • corticotropin-releasing factor
  • dynorphin
  • galanin
  • neurotensin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neuropeptide organization of the hypothalamic projection to the parabrachial nucleus in the rat. / Moga, Margaret; Saper, C. B.; Gray, T. S.

In: Journal of Comparative Neurology, Vol. 295, No. 4, 1990, p. 662-682.

Research output: Contribution to journalArticle

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N2 - The hypothalamus is a major source of afferents to the parabrachial nucleus (PB), but the neurotransmitters in this pathway are largely unknown. In this study, we examine the neuropeptide immunoreactivities of neurons in the hypothalamus that project to the PB by using the combined retrograde fluorescence-immunofluorescence method. After injections of the fluorescent tracer blue into the PB, retrogradely labeled neurons were oberved in the paraventricular, dorsomedial, ventromedial, median preoptic, and anteroventral periventricular hypothalamic nuclei; in the dorsal, retrochiasmatic, and lateral hypothalamic areas; and in the medial and lateral preoptic areas. Our results show that at least five distinct neuropeptide-immunoreactive cell populations in the hypothalamus project to the PB. In the perifornical lateral hypothalamus, many neurotensin (NT)-, corticotropin-releasing factor-, dynorphin (DYN)-, angiotensin II (AII)-, and galanin-like immunoreactive (-ir) neurons were retrogradely labeled. A cluster of retrogradely labeled neurons in the juxtacapsular lateral hypothalamus stained with an antiserum against α-melanocyte stimulating hormone (αMSH). Over 50% of the retrogradely labeled cells in the arcuate nucleus were adrenocorticotropin (ACTH)-or αMSH-ir. Many αMSH- and ACTH-ir, and a few DYN-, NT- and AII-ir neurons in the retrochiasmatic area were retrogradely labeled. Only small numbers of double-labeled neurons were found in the paraventricular nucleus, and, of these, enkephalin-ir and dyhorphin-ir neurons were the most common. Somatostatin-ir cells in the hypothalamus were rarely double-labeled. The chemical coding of these hypothalamic projections to the PB may provide important clues to the functional organization of these descending pathways.

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