Neuropeptide Y Is Produced by Adipose Tissue Macrophages and Regulates Obesity-Induced Inflammation

Kanakadurga Singer, David L. Morris, Kelsie E. Oatmen, Tianyi Wang, Jennifer DelProposto, Taleen Mergian, Kae Won Cho, Carey N. Lumeng

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Neuropeptide Y (NPY) is induced in peripheral tissues such as adipose tissue with obesity. The mechanism and function of NPY induction in fat are unclear. Given the evidence that NPY can modulate inflammation, we examined the hypothesis that NPY regulates the function of adipose tissue macrophages (ATMs) in response to dietary obesity in mice. NPY was induced by dietary obesity in the stromal vascular cells of visceral fat depots from mice. Surprisingly, the induction of Npy was limited to purified ATMs from obese mice. Significant basal production of NPY was observed in cultured bone marrow derived macrophage and dendritic cells (DCs) and was increased with LPS stimulation. In vitro, addition of NPY to myeloid cells had minimal effects on their activation profiles. NPY receptor inhibition promoted DC maturation and the production of IL-6 and TNFα suggesting an anti-inflammatory function for NPY signaling in DCs. Consistent with this, NPY injection into lean mice decreased the quantity of M1-like CD11c+ ATMs and suppressed Ly6chi monocytes. BM chimeras generated from Npy-/- donors demonstrated that hematopoietic NPY contributes to the obesity-induced induction of Npy in fat. In addition, loss of Npy expression from hematopoietic cells led to an increase in CD11c+ ATMs in visceral fat with high fat diet feeding. Overall, our studies suggest that NPY is produced by a range of myeloid cells and that obesity activates the production of NPY in adipose tissue macrophages with autocrine and paracrine effects.

Original languageEnglish (US)
Article numbere57929
JournalPLoS One
Volume8
Issue number3
DOIs
StatePublished - Mar 5 2013
Externally publishedYes

Fingerprint

neuropeptide Y
Macrophages
Neuropeptide Y
adipose tissue
Adipose Tissue
macrophages
obesity
Obesity
inflammation
Tissue
Inflammation
Fats
dendritic cells
Dendritic Cells
Intra-Abdominal Fat
visceral fat
Myeloid Cells
mice
Neuropeptide Y Receptors
cells

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Singer, K., Morris, D. L., Oatmen, K. E., Wang, T., DelProposto, J., Mergian, T., ... Lumeng, C. N. (2013). Neuropeptide Y Is Produced by Adipose Tissue Macrophages and Regulates Obesity-Induced Inflammation. PLoS One, 8(3), [e57929]. https://doi.org/10.1371/journal.pone.0057929

Neuropeptide Y Is Produced by Adipose Tissue Macrophages and Regulates Obesity-Induced Inflammation. / Singer, Kanakadurga; Morris, David L.; Oatmen, Kelsie E.; Wang, Tianyi; DelProposto, Jennifer; Mergian, Taleen; Cho, Kae Won; Lumeng, Carey N.

In: PLoS One, Vol. 8, No. 3, e57929, 05.03.2013.

Research output: Contribution to journalArticle

Singer, K, Morris, DL, Oatmen, KE, Wang, T, DelProposto, J, Mergian, T, Cho, KW & Lumeng, CN 2013, 'Neuropeptide Y Is Produced by Adipose Tissue Macrophages and Regulates Obesity-Induced Inflammation', PLoS One, vol. 8, no. 3, e57929. https://doi.org/10.1371/journal.pone.0057929
Singer K, Morris DL, Oatmen KE, Wang T, DelProposto J, Mergian T et al. Neuropeptide Y Is Produced by Adipose Tissue Macrophages and Regulates Obesity-Induced Inflammation. PLoS One. 2013 Mar 5;8(3). e57929. https://doi.org/10.1371/journal.pone.0057929
Singer, Kanakadurga ; Morris, David L. ; Oatmen, Kelsie E. ; Wang, Tianyi ; DelProposto, Jennifer ; Mergian, Taleen ; Cho, Kae Won ; Lumeng, Carey N. / Neuropeptide Y Is Produced by Adipose Tissue Macrophages and Regulates Obesity-Induced Inflammation. In: PLoS One. 2013 ; Vol. 8, No. 3.
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