Neuropeptide Y-mediated sex-and afferent-specific neurotransmissions contribute to sexual dimorphism of baroreflex afferent function

Yang Liu, Di Wu, Mei Yu Qu, Jian Li He, Mei Yuan, Miao Zhao, Jian Xin Wang, Jian He, Lu Qi Wang, Xin Jing Guo, Meng Zuo, Shu Yang Zhao, Mei Na Ma, Jun Nan Li, Weinian Shou, Guo Fen Qiao, Bai Yan Li

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Molecular and cellular mechanisms of neuropeptide-Y (NPY)-mediated gender-difference in blood pressure (BP) regulation are largely unknown. Methods: Baroreceptor sensitivity (BRS) was evaluated by measuring the response of BP to phenylephrine/nitroprusside. Serum NPY concentration was determined using ELISA. The mRNA and protein expression of NPY receptors were assessed in tissue and single-cell by RT-PCR, immunoblot, and immunohistochemistry. NPY was injected into the nodose while arterial pressure was monitored. Electrophysiological recordings were performed on nodose neurons from rats by patch-clamp technique. Results: The BRS was higher in female than male and ovariectomized rats, while serum NPY concentration was similar among groups. The sex-difference was detected in Y1R, not Y2R protein expression, however, both were upregulated upon ovariectomy and canceled by estrogen replacement. Immunostaining confirmed Y1R and Y2R expression in myelinated and unmyelinated afferents. Single-cell PCR demonstrated that Y1R expression/distribution was identical between A-and C-types, whereas, expressed level of Y2R was ~15 and ~7 folds higher in Ah-and C-types than A-types despite similar distribution. Activation of Y1R in nodose elevated BP, while activation of Y2R did the opposite. Activation of Y1R did not alter action potential duration (APD) of A-types, but activation of Y2R-and Y1R/Y2R in Ah-and C-types frequency-dependently prolonged APD. N-type ICa was reduced in A-, Ah-and C-types when either Y1R, Y2R, or both were activated. The sex-difference in Y1R expression was also observed in NTS. Conclusions: Sex-and afferent-specific expression of Neuropeptide-Y receptors in baroreflex afferent pathway may contribute to sexual-dimorphic neurocontrol of BP regulation.

Original languageEnglish (US)
Pages (from-to)66135-66148
Number of pages14
JournalOncotarget
Volume7
Issue number40
DOIs
StatePublished - 2016

Fingerprint

Baroreflex
Neuropeptide Y
Synaptic Transmission
Sex Characteristics
Neuropeptide Y Receptors
Blood Pressure
Pressoreceptors
Action Potentials
Afferent Pathways
Polymerase Chain Reaction
Estrogen Replacement Therapy
Nitroprusside
Ovariectomy
Phenylephrine
Patch-Clamp Techniques
Serum
Arterial Pressure
Proteins
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry

Keywords

  • Baroreflex
  • Neuropeptide Y
  • Nodose ganglion
  • Nucleus tractus solitarii
  • Whole-cell patch techniques

ASJC Scopus subject areas

  • Oncology

Cite this

Neuropeptide Y-mediated sex-and afferent-specific neurotransmissions contribute to sexual dimorphism of baroreflex afferent function. / Liu, Yang; Wu, Di; Qu, Mei Yu; He, Jian Li; Yuan, Mei; Zhao, Miao; Wang, Jian Xin; He, Jian; Wang, Lu Qi; Guo, Xin Jing; Zuo, Meng; Zhao, Shu Yang; Ma, Mei Na; Li, Jun Nan; Shou, Weinian; Qiao, Guo Fen; Li, Bai Yan.

In: Oncotarget, Vol. 7, No. 40, 2016, p. 66135-66148.

Research output: Contribution to journalArticle

Liu, Y, Wu, D, Qu, MY, He, JL, Yuan, M, Zhao, M, Wang, JX, He, J, Wang, LQ, Guo, XJ, Zuo, M, Zhao, SY, Ma, MN, Li, JN, Shou, W, Qiao, GF & Li, BY 2016, 'Neuropeptide Y-mediated sex-and afferent-specific neurotransmissions contribute to sexual dimorphism of baroreflex afferent function', Oncotarget, vol. 7, no. 40, pp. 66135-66148. https://doi.org/10.18632/oncotarget.11880
Liu, Yang ; Wu, Di ; Qu, Mei Yu ; He, Jian Li ; Yuan, Mei ; Zhao, Miao ; Wang, Jian Xin ; He, Jian ; Wang, Lu Qi ; Guo, Xin Jing ; Zuo, Meng ; Zhao, Shu Yang ; Ma, Mei Na ; Li, Jun Nan ; Shou, Weinian ; Qiao, Guo Fen ; Li, Bai Yan. / Neuropeptide Y-mediated sex-and afferent-specific neurotransmissions contribute to sexual dimorphism of baroreflex afferent function. In: Oncotarget. 2016 ; Vol. 7, No. 40. pp. 66135-66148.
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abstract = "Background: Molecular and cellular mechanisms of neuropeptide-Y (NPY)-mediated gender-difference in blood pressure (BP) regulation are largely unknown. Methods: Baroreceptor sensitivity (BRS) was evaluated by measuring the response of BP to phenylephrine/nitroprusside. Serum NPY concentration was determined using ELISA. The mRNA and protein expression of NPY receptors were assessed in tissue and single-cell by RT-PCR, immunoblot, and immunohistochemistry. NPY was injected into the nodose while arterial pressure was monitored. Electrophysiological recordings were performed on nodose neurons from rats by patch-clamp technique. Results: The BRS was higher in female than male and ovariectomized rats, while serum NPY concentration was similar among groups. The sex-difference was detected in Y1R, not Y2R protein expression, however, both were upregulated upon ovariectomy and canceled by estrogen replacement. Immunostaining confirmed Y1R and Y2R expression in myelinated and unmyelinated afferents. Single-cell PCR demonstrated that Y1R expression/distribution was identical between A-and C-types, whereas, expressed level of Y2R was ~15 and ~7 folds higher in Ah-and C-types than A-types despite similar distribution. Activation of Y1R in nodose elevated BP, while activation of Y2R did the opposite. Activation of Y1R did not alter action potential duration (APD) of A-types, but activation of Y2R-and Y1R/Y2R in Ah-and C-types frequency-dependently prolonged APD. N-type ICa was reduced in A-, Ah-and C-types when either Y1R, Y2R, or both were activated. The sex-difference in Y1R expression was also observed in NTS. Conclusions: Sex-and afferent-specific expression of Neuropeptide-Y receptors in baroreflex afferent pathway may contribute to sexual-dimorphic neurocontrol of BP regulation.",
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author = "Yang Liu and Di Wu and Qu, {Mei Yu} and He, {Jian Li} and Mei Yuan and Miao Zhao and Wang, {Jian Xin} and Jian He and Wang, {Lu Qi} and Guo, {Xin Jing} and Meng Zuo and Zhao, {Shu Yang} and Ma, {Mei Na} and Li, {Jun Nan} and Weinian Shou and Qiao, {Guo Fen} and Li, {Bai Yan}",
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T1 - Neuropeptide Y-mediated sex-and afferent-specific neurotransmissions contribute to sexual dimorphism of baroreflex afferent function

AU - Liu, Yang

AU - Wu, Di

AU - Qu, Mei Yu

AU - He, Jian Li

AU - Yuan, Mei

AU - Zhao, Miao

AU - Wang, Jian Xin

AU - He, Jian

AU - Wang, Lu Qi

AU - Guo, Xin Jing

AU - Zuo, Meng

AU - Zhao, Shu Yang

AU - Ma, Mei Na

AU - Li, Jun Nan

AU - Shou, Weinian

AU - Qiao, Guo Fen

AU - Li, Bai Yan

PY - 2016

Y1 - 2016

N2 - Background: Molecular and cellular mechanisms of neuropeptide-Y (NPY)-mediated gender-difference in blood pressure (BP) regulation are largely unknown. Methods: Baroreceptor sensitivity (BRS) was evaluated by measuring the response of BP to phenylephrine/nitroprusside. Serum NPY concentration was determined using ELISA. The mRNA and protein expression of NPY receptors were assessed in tissue and single-cell by RT-PCR, immunoblot, and immunohistochemistry. NPY was injected into the nodose while arterial pressure was monitored. Electrophysiological recordings were performed on nodose neurons from rats by patch-clamp technique. Results: The BRS was higher in female than male and ovariectomized rats, while serum NPY concentration was similar among groups. The sex-difference was detected in Y1R, not Y2R protein expression, however, both were upregulated upon ovariectomy and canceled by estrogen replacement. Immunostaining confirmed Y1R and Y2R expression in myelinated and unmyelinated afferents. Single-cell PCR demonstrated that Y1R expression/distribution was identical between A-and C-types, whereas, expressed level of Y2R was ~15 and ~7 folds higher in Ah-and C-types than A-types despite similar distribution. Activation of Y1R in nodose elevated BP, while activation of Y2R did the opposite. Activation of Y1R did not alter action potential duration (APD) of A-types, but activation of Y2R-and Y1R/Y2R in Ah-and C-types frequency-dependently prolonged APD. N-type ICa was reduced in A-, Ah-and C-types when either Y1R, Y2R, or both were activated. The sex-difference in Y1R expression was also observed in NTS. Conclusions: Sex-and afferent-specific expression of Neuropeptide-Y receptors in baroreflex afferent pathway may contribute to sexual-dimorphic neurocontrol of BP regulation.

AB - Background: Molecular and cellular mechanisms of neuropeptide-Y (NPY)-mediated gender-difference in blood pressure (BP) regulation are largely unknown. Methods: Baroreceptor sensitivity (BRS) was evaluated by measuring the response of BP to phenylephrine/nitroprusside. Serum NPY concentration was determined using ELISA. The mRNA and protein expression of NPY receptors were assessed in tissue and single-cell by RT-PCR, immunoblot, and immunohistochemistry. NPY was injected into the nodose while arterial pressure was monitored. Electrophysiological recordings were performed on nodose neurons from rats by patch-clamp technique. Results: The BRS was higher in female than male and ovariectomized rats, while serum NPY concentration was similar among groups. The sex-difference was detected in Y1R, not Y2R protein expression, however, both were upregulated upon ovariectomy and canceled by estrogen replacement. Immunostaining confirmed Y1R and Y2R expression in myelinated and unmyelinated afferents. Single-cell PCR demonstrated that Y1R expression/distribution was identical between A-and C-types, whereas, expressed level of Y2R was ~15 and ~7 folds higher in Ah-and C-types than A-types despite similar distribution. Activation of Y1R in nodose elevated BP, while activation of Y2R did the opposite. Activation of Y1R did not alter action potential duration (APD) of A-types, but activation of Y2R-and Y1R/Y2R in Ah-and C-types frequency-dependently prolonged APD. N-type ICa was reduced in A-, Ah-and C-types when either Y1R, Y2R, or both were activated. The sex-difference in Y1R expression was also observed in NTS. Conclusions: Sex-and afferent-specific expression of Neuropeptide-Y receptors in baroreflex afferent pathway may contribute to sexual-dimorphic neurocontrol of BP regulation.

KW - Baroreflex

KW - Neuropeptide Y

KW - Nodose ganglion

KW - Nucleus tractus solitarii

KW - Whole-cell patch techniques

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