Neuroregeneration and voiding behavior patterns after pudendal nerve crush in female rats

K. Sakamoto, G. M. Smith, P. D. Storer, Kathryn Jones, M. S. Damaser

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Since the pudendal nerve innervates the external urethral sphincter, pudendal nerve injury and resultant neuroregeneration should affect voiding behavior. In this study, neuroregenerative activity of pudendal nerve was correlated to the changes in urinary behavior in female rats. Eighteen female rats underwent bilateral pudendal nerve crush, and 17 to 21 age-matched rats were used as unoperated controls. Urinary volume and frequency were recorded 6 and 13 days post-operatively (dpo). Initiation of pudendal nerve regeneration was indicated by an upregulation of β(II) tubulin mRNA in the dorsolateral motoneurons (DLM), as measured at 7 and 14 dpo by in situ hybridization with radio-labeled ̄(II) tubulin cDNA. At 6 dpo, mean volume voided by the crush group was significantly decreased compared to the control group during the light cycle (P <0.05). At 7 dpo, the DLM mRNA level was significantly increased in the nerve crush group compared to the control group (P <0.05). At 13 dpo, there were no differences in volume or frequency between the two groups, suggesting a return to normal voiding behavior. At 14 dpo, there was no significant difference in DLM mRNA levels between crush and control groups. Initiation of nerve regeneration occurs before normalization of voiding behavior after pudendal nerve crush. This data suggest that treatments to accelerate nerve regeneration would improve functional recovery of neurologically based incontinence. (C) 2000 Wiley-Liss. Inc.

Original languageEnglish (US)
Pages (from-to)311-321
Number of pages11
JournalNeurourology and Urodynamics
Volume19
Issue number3
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Nerve Crush
Pudendal Nerve
Nerve Regeneration
Motor Neurons
Tubulin
Control Groups
Messenger RNA
Photoperiod
Urethra
Radio
In Situ Hybridization
Up-Regulation
Complementary DNA
Wounds and Injuries

Keywords

  • Injury
  • Nerve
  • Nerve regeneration
  • Pudendal nerve
  • Rat
  • Voiding

ASJC Scopus subject areas

  • Clinical Neurology
  • Nephrology
  • Urology

Cite this

Neuroregeneration and voiding behavior patterns after pudendal nerve crush in female rats. / Sakamoto, K.; Smith, G. M.; Storer, P. D.; Jones, Kathryn; Damaser, M. S.

In: Neurourology and Urodynamics, Vol. 19, No. 3, 2000, p. 311-321.

Research output: Contribution to journalArticle

Sakamoto, K. ; Smith, G. M. ; Storer, P. D. ; Jones, Kathryn ; Damaser, M. S. / Neuroregeneration and voiding behavior patterns after pudendal nerve crush in female rats. In: Neurourology and Urodynamics. 2000 ; Vol. 19, No. 3. pp. 311-321.
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