Neurosurgical Flexible Probe Microscopy with Enhanced Architectural and Cytological Detail

Hany Osman, Deena Elsahy, Veronika Slivova, Corey Thompson, Joseph Georges, Steven Yocom, Aaron Cohen-Gadol

Research output: Contribution to journalArticle

Abstract

Background: Microscopic delineation and clearance of tumor cells at neurosurgical excision margins potentially reduce tumor recurrence and increase patient survival. Probe-based in vivo fluorescence microscopy technologies are promising for neurosurgical in vivo microscopy. Objective: We sought to demonstrate a flexible fiberoptic epifluorescence microscope capable of enhanced architectural and cytological imaging for in vivo microscopy during neurosurgical procedures. Methods: Eighteen specimens were procured from neurosurgical procedures. These specimens were stained with acridine orange and imaged with a 3-dimensional (3D)-printed epifluorescent microscope that incorporates a flexible fiberoptic probe. Still images and video sequence frames were processed using frame alignment, signal projection, and pseudo-coloring, resulting in resolution enhancement and an increased field of view. Results: Images produced displayed good nuclear contrast and architectural detail. Grade 1 meningiomas demonstrated 3D chords and whorls. Low-grade meningothelial nuclei showed streaming and displayed regularity in size, shape, and distribution. Oligodendrogliomas showed regular round nuclei and a variably staining background. Glioblastomas showed high degrees of nuclear pleomorphism and disarray. Mitoses, vascular proliferation, and necrosis were evident. Conclusions: We demonstrate the utility of a 3D-printed, flexible probe microscope for high-resolution microscopic imaging with increased architectural detail. Enhanced in vivo imaging using this device may improve our ability to detect and decrease microscopic tumor burden at excision margins during neurosurgical procedures.

Original languageEnglish (US)
JournalWorld Neurosurgery
DOIs
StatePublished - Jan 1 2019

Fingerprint

Neurosurgical Procedures
Microscopy
Oligodendroglioma
Acridine Orange
Meningioma
Glioblastoma
Tumor Burden
Fluorescence Microscopy
Mitosis
Blood Vessels
Neoplasms
Necrosis
Staining and Labeling
Technology
Recurrence
Equipment and Supplies
Survival
Intravital Microscopy
Margins of Excision

Keywords

  • Ex vivo
  • Glioma
  • In vivo
  • Microscopy
  • Neurosurgery
  • Oncology

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

Cite this

Neurosurgical Flexible Probe Microscopy with Enhanced Architectural and Cytological Detail. / Osman, Hany; Elsahy, Deena; Slivova, Veronika; Thompson, Corey; Georges, Joseph; Yocom, Steven; Cohen-Gadol, Aaron.

In: World Neurosurgery, 01.01.2019.

Research output: Contribution to journalArticle

Osman, Hany ; Elsahy, Deena ; Slivova, Veronika ; Thompson, Corey ; Georges, Joseph ; Yocom, Steven ; Cohen-Gadol, Aaron. / Neurosurgical Flexible Probe Microscopy with Enhanced Architectural and Cytological Detail. In: World Neurosurgery. 2019.
@article{1f96939c804143bf9117671b4a89ac6d,
title = "Neurosurgical Flexible Probe Microscopy with Enhanced Architectural and Cytological Detail",
abstract = "Background: Microscopic delineation and clearance of tumor cells at neurosurgical excision margins potentially reduce tumor recurrence and increase patient survival. Probe-based in vivo fluorescence microscopy technologies are promising for neurosurgical in vivo microscopy. Objective: We sought to demonstrate a flexible fiberoptic epifluorescence microscope capable of enhanced architectural and cytological imaging for in vivo microscopy during neurosurgical procedures. Methods: Eighteen specimens were procured from neurosurgical procedures. These specimens were stained with acridine orange and imaged with a 3-dimensional (3D)-printed epifluorescent microscope that incorporates a flexible fiberoptic probe. Still images and video sequence frames were processed using frame alignment, signal projection, and pseudo-coloring, resulting in resolution enhancement and an increased field of view. Results: Images produced displayed good nuclear contrast and architectural detail. Grade 1 meningiomas demonstrated 3D chords and whorls. Low-grade meningothelial nuclei showed streaming and displayed regularity in size, shape, and distribution. Oligodendrogliomas showed regular round nuclei and a variably staining background. Glioblastomas showed high degrees of nuclear pleomorphism and disarray. Mitoses, vascular proliferation, and necrosis were evident. Conclusions: We demonstrate the utility of a 3D-printed, flexible probe microscope for high-resolution microscopic imaging with increased architectural detail. Enhanced in vivo imaging using this device may improve our ability to detect and decrease microscopic tumor burden at excision margins during neurosurgical procedures.",
keywords = "Ex vivo, Glioma, In vivo, Microscopy, Neurosurgery, Oncology",
author = "Hany Osman and Deena Elsahy and Veronika Slivova and Corey Thompson and Joseph Georges and Steven Yocom and Aaron Cohen-Gadol",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.wneu.2019.05.039",
language = "English (US)",
journal = "World Neurosurgery",
issn = "1878-8750",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Neurosurgical Flexible Probe Microscopy with Enhanced Architectural and Cytological Detail

AU - Osman, Hany

AU - Elsahy, Deena

AU - Slivova, Veronika

AU - Thompson, Corey

AU - Georges, Joseph

AU - Yocom, Steven

AU - Cohen-Gadol, Aaron

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: Microscopic delineation and clearance of tumor cells at neurosurgical excision margins potentially reduce tumor recurrence and increase patient survival. Probe-based in vivo fluorescence microscopy technologies are promising for neurosurgical in vivo microscopy. Objective: We sought to demonstrate a flexible fiberoptic epifluorescence microscope capable of enhanced architectural and cytological imaging for in vivo microscopy during neurosurgical procedures. Methods: Eighteen specimens were procured from neurosurgical procedures. These specimens were stained with acridine orange and imaged with a 3-dimensional (3D)-printed epifluorescent microscope that incorporates a flexible fiberoptic probe. Still images and video sequence frames were processed using frame alignment, signal projection, and pseudo-coloring, resulting in resolution enhancement and an increased field of view. Results: Images produced displayed good nuclear contrast and architectural detail. Grade 1 meningiomas demonstrated 3D chords and whorls. Low-grade meningothelial nuclei showed streaming and displayed regularity in size, shape, and distribution. Oligodendrogliomas showed regular round nuclei and a variably staining background. Glioblastomas showed high degrees of nuclear pleomorphism and disarray. Mitoses, vascular proliferation, and necrosis were evident. Conclusions: We demonstrate the utility of a 3D-printed, flexible probe microscope for high-resolution microscopic imaging with increased architectural detail. Enhanced in vivo imaging using this device may improve our ability to detect and decrease microscopic tumor burden at excision margins during neurosurgical procedures.

AB - Background: Microscopic delineation and clearance of tumor cells at neurosurgical excision margins potentially reduce tumor recurrence and increase patient survival. Probe-based in vivo fluorescence microscopy technologies are promising for neurosurgical in vivo microscopy. Objective: We sought to demonstrate a flexible fiberoptic epifluorescence microscope capable of enhanced architectural and cytological imaging for in vivo microscopy during neurosurgical procedures. Methods: Eighteen specimens were procured from neurosurgical procedures. These specimens were stained with acridine orange and imaged with a 3-dimensional (3D)-printed epifluorescent microscope that incorporates a flexible fiberoptic probe. Still images and video sequence frames were processed using frame alignment, signal projection, and pseudo-coloring, resulting in resolution enhancement and an increased field of view. Results: Images produced displayed good nuclear contrast and architectural detail. Grade 1 meningiomas demonstrated 3D chords and whorls. Low-grade meningothelial nuclei showed streaming and displayed regularity in size, shape, and distribution. Oligodendrogliomas showed regular round nuclei and a variably staining background. Glioblastomas showed high degrees of nuclear pleomorphism and disarray. Mitoses, vascular proliferation, and necrosis were evident. Conclusions: We demonstrate the utility of a 3D-printed, flexible probe microscope for high-resolution microscopic imaging with increased architectural detail. Enhanced in vivo imaging using this device may improve our ability to detect and decrease microscopic tumor burden at excision margins during neurosurgical procedures.

KW - Ex vivo

KW - Glioma

KW - In vivo

KW - Microscopy

KW - Neurosurgery

KW - Oncology

UR - http://www.scopus.com/inward/record.url?scp=85066497070&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85066497070&partnerID=8YFLogxK

U2 - 10.1016/j.wneu.2019.05.039

DO - 10.1016/j.wneu.2019.05.039

M3 - Article

C2 - 31100529

AN - SCOPUS:85066497070

JO - World Neurosurgery

JF - World Neurosurgery

SN - 1878-8750

ER -