Neutral-alkaline proteolytic activity in rat cardiac muscle cells

Cathy J. Beinlich, Michael G. Clark, Edward E. McKee, Jeffrey A. Lins, Howard E. Morgan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Homogenates that were prepared from isolated heart muscle cells were found to have reduced ability to degrade endogenous proteins to free amino acids at alkaline pH in comparison to homogenates of whole hearts. The decrease in alkaline proteolytic activity was the result of loss from the particulate fraction. The heart muscle cell preparations retained considerable proteolytic activity at neutral-alkaline pH as indicated by the hydrolysis of [3H]acetylcasein and [14C]protein from rat heart to acid soluble products. In both cases, the decrease in activity was associated with the particulate fraction and appeared to be the result of the absence of mast cells. The extractable activity of control hearts and heart muscle cells eluted from DEAE-cellulose columns as two peaks of caseinolytic activity. The first peak eluted with 0.07 to 0.12 m NaCl and included a thiol protease. The second peak eluted with 0.25 m NaCl and was seen only in the presence of 5 mm CaCl2. The specific activity of the Ca2+-dependent protease was reduced 50% in muscle cell preparations as compared to control heart.

Original languageEnglish (US)
Pages (from-to)23-36
Number of pages14
JournalJournal of Molecular and Cellular Cardiology
Volume13
Issue number1
DOIs
StatePublished - 1981
Externally publishedYes

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Cardiac Myocytes
Peptide Hydrolases
DEAE-Cellulose
Sulfhydryl Compounds
Mast Cells
Muscle Cells
Proteins
Hydrolysis
Amino Acids
Acids

Keywords

  • Casein hydrolysis
  • Heart muscle cells
  • Mast cells
  • Particulate proteases
  • Soluble proteases

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Neutral-alkaline proteolytic activity in rat cardiac muscle cells. / Beinlich, Cathy J.; Clark, Michael G.; McKee, Edward E.; Lins, Jeffrey A.; Morgan, Howard E.

In: Journal of Molecular and Cellular Cardiology, Vol. 13, No. 1, 1981, p. 23-36.

Research output: Contribution to journalArticle

Beinlich, Cathy J. ; Clark, Michael G. ; McKee, Edward E. ; Lins, Jeffrey A. ; Morgan, Howard E. / Neutral-alkaline proteolytic activity in rat cardiac muscle cells. In: Journal of Molecular and Cellular Cardiology. 1981 ; Vol. 13, No. 1. pp. 23-36.
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