New approaches to analyzing the site selectivities and crystal structures of DNA targeted metal complexes: Analysis of the antitumor agent bleomycin

Eric C. Long, Millie Georgiadis, Kristie D. Goodwin, Mark A. Lewis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

New approaches to determining the site-selectivities of DNA targeted metal complexes and their DNA-bound crystal structures are described as applied to bleomycin, a metal binding antitumor agent. These approaches include a fluorescent intercalator displacement assay and a host-guest DNA-ligand co-crystallization strategy. The application of these approaches, respectively, confirmed that the binding site-selectivities of Co(III)· and Fe(III)·bleomycin parallel the established cleavage selectivity of this drug and led to the first crystal structures of duplex DNA-bound Co(III)·bleomycin.

Original languageEnglish
Title of host publicationACS Symposium Series
PublisherAmerican Chemical Society
Pages63-80
Number of pages18
Volume1012
ISBN (Print)9780841269750
DOIs
StatePublished - May 21 2009

Publication series

NameACS Symposium Series
Volume1012
ISSN (Print)00976156
ISSN (Electronic)19475918

Fingerprint

Coordination Complexes
Bleomycin
Metal complexes
Antineoplastic Agents
DNA
Crystal structure
Intercalating Agents
Binding sites
Crystallization
Assays
Metals
Binding Sites
Ligands
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Long, E. C., Georgiadis, M., Goodwin, K. D., & Lewis, M. A. (2009). New approaches to analyzing the site selectivities and crystal structures of DNA targeted metal complexes: Analysis of the antitumor agent bleomycin. In ACS Symposium Series (Vol. 1012, pp. 63-80). (ACS Symposium Series; Vol. 1012). American Chemical Society. https://doi.org/10.1021/bk-2009-1012.ch005

New approaches to analyzing the site selectivities and crystal structures of DNA targeted metal complexes : Analysis of the antitumor agent bleomycin. / Long, Eric C.; Georgiadis, Millie; Goodwin, Kristie D.; Lewis, Mark A.

ACS Symposium Series. Vol. 1012 American Chemical Society, 2009. p. 63-80 (ACS Symposium Series; Vol. 1012).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Long, EC, Georgiadis, M, Goodwin, KD & Lewis, MA 2009, New approaches to analyzing the site selectivities and crystal structures of DNA targeted metal complexes: Analysis of the antitumor agent bleomycin. in ACS Symposium Series. vol. 1012, ACS Symposium Series, vol. 1012, American Chemical Society, pp. 63-80. https://doi.org/10.1021/bk-2009-1012.ch005
Long EC, Georgiadis M, Goodwin KD, Lewis MA. New approaches to analyzing the site selectivities and crystal structures of DNA targeted metal complexes: Analysis of the antitumor agent bleomycin. In ACS Symposium Series. Vol. 1012. American Chemical Society. 2009. p. 63-80. (ACS Symposium Series). https://doi.org/10.1021/bk-2009-1012.ch005
Long, Eric C. ; Georgiadis, Millie ; Goodwin, Kristie D. ; Lewis, Mark A. / New approaches to analyzing the site selectivities and crystal structures of DNA targeted metal complexes : Analysis of the antitumor agent bleomycin. ACS Symposium Series. Vol. 1012 American Chemical Society, 2009. pp. 63-80 (ACS Symposium Series).
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