New approaches to analyzing the site selectivities and crystal structures of DNA targeted metal complexes: Analysis of the antitumor agent bleomycin

Eric C. Long, Millie M. Georgiadis, Kristie D. Goodwin, Mark A. Lewis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

New approaches to determining the site-selectivities of DNA targeted metal complexes and their DNA-bound crystal structures are described as applied to bleomycin, a metal binding antitumor agent. These approaches include a fluorescent intercalator displacement assay and a host-guest DNA-ligand co-crystallization strategy. The application of these approaches, respectively, confirmed that the binding site-selectivities of Co(III)· and Fe(III)·bleomycin parallel the established cleavage selectivity of this drug and led to the first crystal structures of duplex DNA-bound Co(III)·bleomycin.

Original languageEnglish (US)
Title of host publicationBioinorganic Chemistry
Subtitle of host publicationCellular Systems and Synthetic Models
PublisherAmerican Chemical Society
Pages63-80
Number of pages18
ISBN (Print)9780841269750
DOIs
StatePublished - May 21 2009

Publication series

NameACS Symposium Series
Volume1012
ISSN (Print)0097-6156
ISSN (Electronic)1947-5918

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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