New approaches to analyzing the site selectivities and crystal structures of DNA targeted metal complexes: Analysis of the antitumor agent bleomycin

Eric C. Long, Millie M. Georgiadis, Kristie D. Goodwin, Mark A. Lewis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

New approaches to determining the site-selectivities of DNA targeted metal complexes and their DNA-bound crystal structures are described as applied to bleomycin, a metal binding antitumor agent. These approaches include a fluorescent intercalator displacement assay and a host-guest DNA-ligand co-crystallization strategy. The application of these approaches, respectively, confirmed that the binding site-selectivities of Co(III)· and Fe(III)·bleomycin parallel the established cleavage selectivity of this drug and led to the first crystal structures of duplex DNA-bound Co(III)·bleomycin.

Original languageEnglish (US)
Title of host publicationBioinorganic Chemistry
Subtitle of host publicationCellular Systems and Synthetic Models
PublisherAmerican Chemical Society
Pages63-80
Number of pages18
ISBN (Print)9780841269750
DOIs
StatePublished - May 21 2009

Publication series

NameACS Symposium Series
Volume1012
ISSN (Print)0097-6156
ISSN (Electronic)1947-5918

Fingerprint

Coordination Complexes
Bleomycin
Metal complexes
Antineoplastic Agents
DNA
Crystal structure
Intercalating Agents
Binding sites
Crystallization
Assays
Metals
Binding Sites
Ligands
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Long, E. C., Georgiadis, M. M., Goodwin, K. D., & Lewis, M. A. (2009). New approaches to analyzing the site selectivities and crystal structures of DNA targeted metal complexes: Analysis of the antitumor agent bleomycin. In Bioinorganic Chemistry: Cellular Systems and Synthetic Models (pp. 63-80). (ACS Symposium Series; Vol. 1012). American Chemical Society. https://doi.org/10.1021/bk-2009-1012.ch005

New approaches to analyzing the site selectivities and crystal structures of DNA targeted metal complexes : Analysis of the antitumor agent bleomycin. / Long, Eric C.; Georgiadis, Millie M.; Goodwin, Kristie D.; Lewis, Mark A.

Bioinorganic Chemistry: Cellular Systems and Synthetic Models. American Chemical Society, 2009. p. 63-80 (ACS Symposium Series; Vol. 1012).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Long, EC, Georgiadis, MM, Goodwin, KD & Lewis, MA 2009, New approaches to analyzing the site selectivities and crystal structures of DNA targeted metal complexes: Analysis of the antitumor agent bleomycin. in Bioinorganic Chemistry: Cellular Systems and Synthetic Models. ACS Symposium Series, vol. 1012, American Chemical Society, pp. 63-80. https://doi.org/10.1021/bk-2009-1012.ch005
Long EC, Georgiadis MM, Goodwin KD, Lewis MA. New approaches to analyzing the site selectivities and crystal structures of DNA targeted metal complexes: Analysis of the antitumor agent bleomycin. In Bioinorganic Chemistry: Cellular Systems and Synthetic Models. American Chemical Society. 2009. p. 63-80. (ACS Symposium Series). https://doi.org/10.1021/bk-2009-1012.ch005
Long, Eric C. ; Georgiadis, Millie M. ; Goodwin, Kristie D. ; Lewis, Mark A. / New approaches to analyzing the site selectivities and crystal structures of DNA targeted metal complexes : Analysis of the antitumor agent bleomycin. Bioinorganic Chemistry: Cellular Systems and Synthetic Models. American Chemical Society, 2009. pp. 63-80 (ACS Symposium Series).
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