New human gene encoding a positive modulator of HIV Tat-mediated transactivation

Hiroshi Shibuya, Kenji Irie, Jun Ninomiya-Tsuji, Mark Goebl, Tadatsugu Taniguchi, Kunihiro Matsumoto

Research output: Contribution to journalArticle

138 Scopus citations

Abstract

The human immunodeficiency virus-1 (HIV-1) protein Tat is a potent activator of virus gene expression1,2. Tat functions through a sequence known as TAR, located immediately downstream of the transcription start site in the long terminal repeat3-5. Several observations suggest that cellular factors cooperate with Tat in the overall transactivating process. We have isolated a human complementary DNA from the new gene MSS1, which may encode such a cellular factor, by transcomplementation of a yeast sgv1- mutant. The MSS1 protein shares 42% sequence identity with the human TBP-1 protein, which binds Tat in vitro and suppresses Tat-mediated transactivation in vivo (ref. 6). We report here that the levels of HIV activation by Tat correlate with endogenous levels of MSS1 messenger RNA. Furthermore, we provide evidence that expression of MSS1 enhances the Tat-mediated transactivation. Our results suggest that MSS1 has a key role in activation of HIV genes regulated by Tat.

Original languageEnglish (US)
Pages (from-to)700-702
Number of pages3
JournalNature
Volume357
Issue number6380
DOIs
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • General

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