New techniques for the separation and measurement of the calcium fractions of normal human serum

W. G. Robertson, M. Peacock

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

A method is described for the production of serum ultrafiltrates through a cellophane membrane under controlled conditions of temperature and pH. The method has been tested for reproducibility and the conditions affecting the ultrafiltration of calcium examined. The relationship between ultrafiltrable and total serum calcium has been studied and the ultrafiltrable fractions of calcium, magnesium and phosphorus determined in the serum of normal human subjects. A potentiometric method for the measurement of ionised calcium in serum ultrafiltrate is also described using a specific divalent cation electrode, and the values obtained in normal human subjects reported.

Original languageEnglish (US)
Pages (from-to)315-326
Number of pages12
JournalClinica Chimica Acta
Volume20
Issue number2
DOIs
StatePublished - May 1968

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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