NHE-1 protein in vascular smooth muscle and lymphocytes from the spontaneously hypertensive rat

Michael S. LaPointe, Minghao Ye, Robert Bacallao, Daniel Batlle

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The present study examined the abundance of NHE-1 protein in cultured vascular smooth muscle cells (VSMCs), freshly isolated thymocytes, and fresh aortic tissue from spontaneously hypertensive rats (SHRs) and age-matched Wistar-Kyoto (WKY) rats. Two sets of affinity-purified anti-bodies (Ab((765- 778)) and Ab((698-711))) against different epitopes of the NHE-1 isoform of the Na+-H+ antiporter were used. Each set of antibodies recognized a major protein band at 105 to 110 kD that was more abundant in protein lysates prepared from cultured VSMCs from the SHR than those from WKY rats (Ab((765- 778)) 0.047±0.011 vs 0.010±0.002 O.D. units/10 μg protein, P+-H+ antiporter as compared to those from WKY rats (17.93±2.07 vs 8.16±1.05 mmol H+/min, P+-H+ antiporter in cultured VSMCs from the SHR, compared to those from WKY rats, is due, at least in part, to increased levels of NHE-1 protein.

Original languageEnglish (US)
Pages (from-to)880-885
Number of pages6
JournalHypertension
Volume30
Issue number4
StatePublished - Oct 1997
Externally publishedYes

Fingerprint

Inbred WKY Rats
Inbred SHR Rats
Vascular Smooth Muscle
Lymphocytes
Smooth Muscle Myocytes
Antiporters
Sodium-Hydrogen Antiporter
Proteins
Thymocytes
Epitopes
Protein Isoforms
growth factor-activatable Na-H exchanger NHE-1
Antibodies

Keywords

  • Muscle, smooth
  • NHE-1
  • Rats, inbred SHR
  • Sodium-hydrogen antiporter
  • Western blot

ASJC Scopus subject areas

  • Internal Medicine

Cite this

NHE-1 protein in vascular smooth muscle and lymphocytes from the spontaneously hypertensive rat. / LaPointe, Michael S.; Ye, Minghao; Bacallao, Robert; Batlle, Daniel.

In: Hypertension, Vol. 30, No. 4, 10.1997, p. 880-885.

Research output: Contribution to journalArticle

LaPointe, Michael S. ; Ye, Minghao ; Bacallao, Robert ; Batlle, Daniel. / NHE-1 protein in vascular smooth muscle and lymphocytes from the spontaneously hypertensive rat. In: Hypertension. 1997 ; Vol. 30, No. 4. pp. 880-885.
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