Nicotine and lipopolysaccharide affect cytokine expression from gingival fibroblasts

Amjad Almasri, Kessiri Wisithphrom, L. Windsor, Byron Olson

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Background: This in vitro study investigated the influence of nicotine, lipopolysaccharide (LPS), and a combination of both agents on cytokine expression from human gingival fibroblasts (HGFs). Methods: HGFs were exposed for 48 hours to 250 μg/ml nicotine, 1 μg/ml Porphyromonas gingivalis LPS, or both. The expression of multiple cytokines was detected in the HGFs conditioned media using cytokine protein arrays. Results: The untreated HGFs expressed several cytokines, which included relatively high levels of interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1 (MCP-1). They also expressed low levels of growth-regulated oncogene (GRO), IL-3, and IL-10. Nicotine had the greatest effect on the expression of GRO-α, IL-7, IL-10, and IL-15 compared to the untreated control. P. gingivalis LPS had the greatest effect on the expression of GRO-α; IL-7; IL-10; and RANTES (regulated on activation, normal T-cell expressed, and presumably secreted) compared to the untreated control. The combination of both agents had the biggest impact on the expression of GRO-α, IL-7, IL-10, IL-15, RANTES, and interferon-γ (IFN-γ) compared to the untreated control. Conclusion: HGFs exposed to nicotine, P. gingivalis LPS, or a combination of both agents increased the expression of multiple cytokines.

Original languageEnglish (US)
Pages (from-to)533-541
Number of pages9
JournalJournal of Periodontology
Volume78
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

Nicotine
Lipopolysaccharides
Oncogenes
Fibroblasts
Interleukin-7
Interleukin-10
Porphyromonas gingivalis
Cytokines
Interleukin-15
Growth
T-Lymphocytes
Protein Array Analysis
Chemokine CCL2
Interleukin-3
Conditioned Culture Medium
Interleukin-8
Interferons
Interleukin-6

Keywords

  • Cytokines
  • Fibroblasts
  • Gingiva
  • Lipopolysaccharide
  • Nicotine

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Nicotine and lipopolysaccharide affect cytokine expression from gingival fibroblasts. / Almasri, Amjad; Wisithphrom, Kessiri; Windsor, L.; Olson, Byron.

In: Journal of Periodontology, Vol. 78, No. 3, 03.2007, p. 533-541.

Research output: Contribution to journalArticle

Almasri, Amjad ; Wisithphrom, Kessiri ; Windsor, L. ; Olson, Byron. / Nicotine and lipopolysaccharide affect cytokine expression from gingival fibroblasts. In: Journal of Periodontology. 2007 ; Vol. 78, No. 3. pp. 533-541.
@article{4eb67116349147c5b6a1ca0a28688e44,
title = "Nicotine and lipopolysaccharide affect cytokine expression from gingival fibroblasts",
abstract = "Background: This in vitro study investigated the influence of nicotine, lipopolysaccharide (LPS), and a combination of both agents on cytokine expression from human gingival fibroblasts (HGFs). Methods: HGFs were exposed for 48 hours to 250 μg/ml nicotine, 1 μg/ml Porphyromonas gingivalis LPS, or both. The expression of multiple cytokines was detected in the HGFs conditioned media using cytokine protein arrays. Results: The untreated HGFs expressed several cytokines, which included relatively high levels of interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1 (MCP-1). They also expressed low levels of growth-regulated oncogene (GRO), IL-3, and IL-10. Nicotine had the greatest effect on the expression of GRO-α, IL-7, IL-10, and IL-15 compared to the untreated control. P. gingivalis LPS had the greatest effect on the expression of GRO-α; IL-7; IL-10; and RANTES (regulated on activation, normal T-cell expressed, and presumably secreted) compared to the untreated control. The combination of both agents had the biggest impact on the expression of GRO-α, IL-7, IL-10, IL-15, RANTES, and interferon-γ (IFN-γ) compared to the untreated control. Conclusion: HGFs exposed to nicotine, P. gingivalis LPS, or a combination of both agents increased the expression of multiple cytokines.",
keywords = "Cytokines, Fibroblasts, Gingiva, Lipopolysaccharide, Nicotine",
author = "Amjad Almasri and Kessiri Wisithphrom and L. Windsor and Byron Olson",
year = "2007",
month = "3",
doi = "10.1902/jop.2007.060296",
language = "English (US)",
volume = "78",
pages = "533--541",
journal = "Journal of Periodontology",
issn = "0022-3492",
publisher = "American Academy of Periodontology",
number = "3",

}

TY - JOUR

T1 - Nicotine and lipopolysaccharide affect cytokine expression from gingival fibroblasts

AU - Almasri, Amjad

AU - Wisithphrom, Kessiri

AU - Windsor, L.

AU - Olson, Byron

PY - 2007/3

Y1 - 2007/3

N2 - Background: This in vitro study investigated the influence of nicotine, lipopolysaccharide (LPS), and a combination of both agents on cytokine expression from human gingival fibroblasts (HGFs). Methods: HGFs were exposed for 48 hours to 250 μg/ml nicotine, 1 μg/ml Porphyromonas gingivalis LPS, or both. The expression of multiple cytokines was detected in the HGFs conditioned media using cytokine protein arrays. Results: The untreated HGFs expressed several cytokines, which included relatively high levels of interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1 (MCP-1). They also expressed low levels of growth-regulated oncogene (GRO), IL-3, and IL-10. Nicotine had the greatest effect on the expression of GRO-α, IL-7, IL-10, and IL-15 compared to the untreated control. P. gingivalis LPS had the greatest effect on the expression of GRO-α; IL-7; IL-10; and RANTES (regulated on activation, normal T-cell expressed, and presumably secreted) compared to the untreated control. The combination of both agents had the biggest impact on the expression of GRO-α, IL-7, IL-10, IL-15, RANTES, and interferon-γ (IFN-γ) compared to the untreated control. Conclusion: HGFs exposed to nicotine, P. gingivalis LPS, or a combination of both agents increased the expression of multiple cytokines.

AB - Background: This in vitro study investigated the influence of nicotine, lipopolysaccharide (LPS), and a combination of both agents on cytokine expression from human gingival fibroblasts (HGFs). Methods: HGFs were exposed for 48 hours to 250 μg/ml nicotine, 1 μg/ml Porphyromonas gingivalis LPS, or both. The expression of multiple cytokines was detected in the HGFs conditioned media using cytokine protein arrays. Results: The untreated HGFs expressed several cytokines, which included relatively high levels of interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1 (MCP-1). They also expressed low levels of growth-regulated oncogene (GRO), IL-3, and IL-10. Nicotine had the greatest effect on the expression of GRO-α, IL-7, IL-10, and IL-15 compared to the untreated control. P. gingivalis LPS had the greatest effect on the expression of GRO-α; IL-7; IL-10; and RANTES (regulated on activation, normal T-cell expressed, and presumably secreted) compared to the untreated control. The combination of both agents had the biggest impact on the expression of GRO-α, IL-7, IL-10, IL-15, RANTES, and interferon-γ (IFN-γ) compared to the untreated control. Conclusion: HGFs exposed to nicotine, P. gingivalis LPS, or a combination of both agents increased the expression of multiple cytokines.

KW - Cytokines

KW - Fibroblasts

KW - Gingiva

KW - Lipopolysaccharide

KW - Nicotine

UR - http://www.scopus.com/inward/record.url?scp=33947669848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947669848&partnerID=8YFLogxK

U2 - 10.1902/jop.2007.060296

DO - 10.1902/jop.2007.060296

M3 - Article

C2 - 17335378

AN - SCOPUS:33947669848

VL - 78

SP - 533

EP - 541

JO - Journal of Periodontology

JF - Journal of Periodontology

SN - 0022-3492

IS - 3

ER -