Nicotine increases the collagen-degrading ability of human gingival fibroblasts

J. Zhou, B. L. Olson, L. Windsor

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background and Objective: The objective of this study was to determine the effects that nicotine and the combination of nicotine and Porphyromonas gingivalis supernatant have on human gingival fibroblast-mediated collagen degradation. Material and Methods: Human gingival fibroblasts were cultured with 25-500 μg/ml of nicotine in collagen-coated six-well plates. On days 1-5, the conditioned media was collected for zymography and western blot analyses of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). The cells were then removed and the collagen cleavage visualized by Coomassie blue staining. To examine the combined effect, 250 μg/ml of nicotine and 10% v/v culture supernatant of P. gingivalis ATCC 33277 were added to the human gingival fibroblasts. The mRNA levels of multiple MMPs and TIMPs were monitored. Results: Nicotine increased the human gingival fibroblast-mediated collagen cleavage. The MMP-14 and MMP-2 produced by the nicotine-treated human gingival fibroblasts more readily underwent zymogen activation. Nicotine treatment resulted in TIMP-2 redistribution to the cell surface. The mRNAs of multiple MMPs and TlMPs were unaltered by nicotine. An additive collagen cleavage effect was observed when the human gingival fibroblasts were treated with both nicotine and P. gingivalis. Conclusion: Nicotine increased human gingival fibroblast-mediated collagen degradation, in part through the activation of membrane-associated MMPs. Nicotine and P. gingivalis had an additive effect on human gingival fibroblast-mediated collagen degradation.

Original languageEnglish (US)
Pages (from-to)228-235
Number of pages8
JournalJournal of Periodontal Research
Volume42
Issue number3
DOIs
StatePublished - Jun 2007

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Nicotine
Collagen
Fibroblasts
Porphyromonas gingivalis
Tissue Inhibitor of Metalloproteinases
Matrix Metalloproteinase Inhibitors
Membrane-Associated Matrix Metalloproteinases
Matrix Metalloproteinase 14
Tissue Inhibitor of Metalloproteinase-2
Messenger RNA
Enzyme Precursors
Matrix Metalloproteinase 2
Conditioned Culture Medium
Matrix Metalloproteinases
Western Blotting
Staining and Labeling

Keywords

  • Collagen-degrading ability
  • Gingival fibroblasts
  • Matrix metalloproteinases
  • Nicotine

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Nicotine increases the collagen-degrading ability of human gingival fibroblasts. / Zhou, J.; Olson, B. L.; Windsor, L.

In: Journal of Periodontal Research, Vol. 42, No. 3, 06.2007, p. 228-235.

Research output: Contribution to journalArticle

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