Nicotine reduces the secretion of Alzheimer's β-amyloid precursor protein containing β-amyloid peptide in the rat without altering synaptic proteins

Debomoy Lahiri, T. Utsuki, D. Chen, Martin Farlow, M. Shoaib, D. K. Ingram, N. H. Greig

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Alzheimer's disease (AD) is characterized by cerebrovascular deposition of the amyloid β-peptide (Aβ), which is derived from a larger β-amyloid precursor protein (βAPP). Altered metabolism of βAPP, resulting in increased Aβ production, appears central in the neuropathology of AD. The processing of the holoprotein βAPP by different "secretase" enzymes results in three major carboxyl-truncated species. One species, which results from the cleavage of βAPP by γ-secretase, is secreted into the cerebrospinal fluid (CSF) and is called sAPPγ as it contains an intact Aβ domain. Moreover, AD is characterized by cholinergic dysfunction and the loss of synaptic proteins. Reports of an inverse relation between nicotine intake, due to cigarette smoking, and the incidence of AD prompted us to investigate the effects of nicotine on βAPP processing and synaptic proteins in rats and in cell culture. Nicotine, 1 and 8 mg/kg/day, doses commensurate with cigarette smoking, and a higher but well tolerated dose, respectively, was administered over 14 days to rats. Levels of sAPP in the CSF sample were evaluated by Western blot analysis. The higher dose significantly increased levels of total sAPP; however, both doses significantly reduced sAPPγ, which contains the amyloidogenic portion of Aβ These actions were blocked by nicotinic receptor antagonism. Nicotinic antagonists alone had no effect on either total sAPP or sAPPγ levels in CSF. Nicotine did not significantly change the intracellular levels of total βAPP in rat brain extracts, which is consistent with neuronal cell culture data. Similarly, levels of vesicular protein, such as synaptophysin, and presynaptic terminal protein SNAP-25 were unaffected by nicotine treatment both in vivo and in cell culture experiments. Taken together, these results suggest that nicotine modifies βAPP processing away from the formation of potentially amyloidogenic products, without altering the levels of synaptic proteins, and that this can potentially offer therapeutic potential for Alzheimer's disease.

Original languageEnglish
Pages (from-to)364-372
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume965
StatePublished - 2002

Fingerprint

Amyloid beta-Protein Precursor
Nicotine
Amyloid
Rats
Alzheimer Disease
Cerebrospinal fluid
Peptides
Cell culture
Cerebrospinal Fluid
Amyloid Precursor Protein Secretases
Proteins
Cell Culture Techniques
Tobacco Products
Synaptosomal-Associated Protein 25
Processing
Smoking
Nicotinic Antagonists
Synaptophysin
Presynaptic Terminals
Nicotinic Receptors

Keywords

  • β-amyloid peptide
  • β-amyloid precursor protein
  • Alzheimer's disease
  • Cholinergic system
  • Nicotine
  • Synaptic proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Nicotine reduces the secretion of Alzheimer's β-amyloid precursor protein containing β-amyloid peptide in the rat without altering synaptic proteins. / Lahiri, Debomoy; Utsuki, T.; Chen, D.; Farlow, Martin; Shoaib, M.; Ingram, D. K.; Greig, N. H.

In: Annals of the New York Academy of Sciences, Vol. 965, 2002, p. 364-372.

Research output: Contribution to journalArticle

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AU - Farlow, Martin

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AU - Greig, N. H.

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