Nitric oxide production by mouse renal tubules can be increased by a sodium-dependent mechanism

Stephen Kempson, Nathan Thompson, Laura Pezzuto, H. Bohlen

Research output: Contribution to journalArticle

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Abstract

Renal tubules process large amounts of NaCl that other investigators indicate increases tubular generation of nitric oxide. We questioned whether medullary or superficial cortical tubules would have the greater increase in nitric oxide concentration, [NO], when stressed by sodium and if the sodium/calcium exchanger was involved. Sodium stress in proximal tubules is due to the large amount of sodium absorbed and medullary tubules exist in a hypertonic sodium environment. To sodium stress the tissue, mouse kidney slices were exposed to monensin to allow passive entry of sodium ions from isotonic media and in separate studies, 400 and 600 mOsm NaCl was used. [NO] was measured with microelectrodes. Monensin (10 μM) caused a sustained increase in medullary and cortical [NO] to ∼180% of control and 400 mOsm NaCl caused a similar initial increase in [NO] that then subsided. 600 mOsm NaCl caused a more sustained increase in [NO] of >250% of control. L-NAME strongly attenuated the increased [NO] during sodium stress. The increase in [NO] during NaCl elevation was due to sodium ions because mannitol hyperosmolarity caused ∼20% of the increase in [NO]. Entry of sodium during NaCl hyperosmolarity was through bumetanide sensitive channels because the drug suppressed increased [NO]. Blockade of the sodium/calcium ion exchanger strongly suppressed the increased [NO] during monensin, to increase sodium entry into cells, and the elevated NaCl concentration. The data support a sodium-NO linkage that increased NO signaling in proportion to sodium stress by cortical tubules and was highly dependent upon sodium-calcium exchange.

Original languageEnglish
Pages (from-to)33-43
Number of pages11
JournalNitric Oxide - Biology and Chemistry
Volume17
Issue number1
DOIs
StatePublished - Aug 2007

Fingerprint

Nitric Oxide
Sodium
Kidney
Monensin
Sodium-Calcium Exchanger
Ions
Bumetanide
Calcium
NG-Nitroarginine Methyl Ester
Ion exchangers
Microelectrodes
Mannitol
Research Personnel
Tissue

Keywords

  • Hyperosmolarity
  • Kidney
  • Monensin
  • Nitric oxide
  • Tubules

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Nitric oxide production by mouse renal tubules can be increased by a sodium-dependent mechanism. / Kempson, Stephen; Thompson, Nathan; Pezzuto, Laura; Bohlen, H.

In: Nitric Oxide - Biology and Chemistry, Vol. 17, No. 1, 08.2007, p. 33-43.

Research output: Contribution to journalArticle

Kempson, Stephen ; Thompson, Nathan ; Pezzuto, Laura ; Bohlen, H. / Nitric oxide production by mouse renal tubules can be increased by a sodium-dependent mechanism. In: Nitric Oxide - Biology and Chemistry. 2007 ; Vol. 17, No. 1. pp. 33-43.
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