Nix is a selective autophagy receptor for mitochondrial clearance

Ivana Novak, Vladimir Kirkin, David G. McEwan, Ji Zhang, Philipp Wild, Alexis Rozenknop, Vladimir Rogov, Frank Löhr, Doris Popovic, Angelo Occhipinti, Andreas S. Reichert, Janos Terzic, Volker Dötsch, Paul A. Ney, Ivan Dikic

Research output: Contribution to journalArticle

695 Scopus citations


Autophagy is the cellular homeostatic pathway that delivers large cytosolic materials for degradation in the lysosome. Recent evidence indicates that autophagy mediates selective removal of protein aggregates, organelles and microbes in cells. Yet, the specificity in targeting a particular substrate to the autophagy pathway remains poorly understood. Here, we show that the mitochondrial protein Nix is a selective autophagy receptor by binding to LC3/GABARAP proteins, ubiquitin-like modifiers that are required for the growth of autophagosomal membranes. In cultured cells, Nix recruits GABARAP-L1 to damaged mitochondria through its amino-terminal LC3-interacting region. Furthermore, ablation of the Nix:LC3/GABARAP interaction retards mitochondrial clearance in maturing murine reticulocytes. Thus, Nix functions as an autophagy receptor, which mediates mitochondrial clearance after mitochondrial damage and during erythrocyte differentiation.

Original languageEnglish (US)
Pages (from-to)45-51
Number of pages7
JournalEMBO Reports
Issue number1
StatePublished - Jan 2010
Externally publishedYes


  • LC3
  • Mitophagy
  • Nix
  • Selective autophagy

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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  • Cite this

    Novak, I., Kirkin, V., McEwan, D. G., Zhang, J., Wild, P., Rozenknop, A., Rogov, V., Löhr, F., Popovic, D., Occhipinti, A., Reichert, A. S., Terzic, J., Dötsch, V., Ney, P. A., & Dikic, I. (2010). Nix is a selective autophagy receptor for mitochondrial clearance. EMBO Reports, 11(1), 45-51.