Nix is a selective autophagy receptor for mitochondrial clearance

Ivana Novak, Vladimir Kirkin, David G. McEwan, Ji Zhang, Philipp Wild, Alexis Rozenknop, Vladimir Rogov, Frank Löhr, Doris Popovic, Angelo Occhipinti, Andreas S. Reichert, Janos Terzic, Volker Dötsch, Paul A. Ney, Ivan Dikic

Research output: Contribution to journalArticle

586 Citations (Scopus)

Abstract

Autophagy is the cellular homeostatic pathway that delivers large cytosolic materials for degradation in the lysosome. Recent evidence indicates that autophagy mediates selective removal of protein aggregates, organelles and microbes in cells. Yet, the specificity in targeting a particular substrate to the autophagy pathway remains poorly understood. Here, we show that the mitochondrial protein Nix is a selective autophagy receptor by binding to LC3/GABARAP proteins, ubiquitin-like modifiers that are required for the growth of autophagosomal membranes. In cultured cells, Nix recruits GABARAP-L1 to damaged mitochondria through its amino-terminal LC3-interacting region. Furthermore, ablation of the Nix:LC3/GABARAP interaction retards mitochondrial clearance in maturing murine reticulocytes. Thus, Nix functions as an autophagy receptor, which mediates mitochondrial clearance after mitochondrial damage and during erythrocyte differentiation.

Original languageEnglish (US)
Pages (from-to)45-51
Number of pages7
JournalEMBO Reports
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2010
Externally publishedYes

Fingerprint

Ubiquitins
Mitochondria
Mitochondrial Proteins
Autophagy
Ablation
Cells
Membranes
Degradation
Substrates
Reticulocytes
Lysosomes
Organelles
Cultured Cells
Erythrocytes
Protein Aggregates
Growth

Keywords

  • GABARAP
  • LC3
  • Mitophagy
  • Nix
  • Selective autophagy

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Novak, I., Kirkin, V., McEwan, D. G., Zhang, J., Wild, P., Rozenknop, A., ... Dikic, I. (2010). Nix is a selective autophagy receptor for mitochondrial clearance. EMBO Reports, 11(1), 45-51. https://doi.org/10.1038/embor.2009.256

Nix is a selective autophagy receptor for mitochondrial clearance. / Novak, Ivana; Kirkin, Vladimir; McEwan, David G.; Zhang, Ji; Wild, Philipp; Rozenknop, Alexis; Rogov, Vladimir; Löhr, Frank; Popovic, Doris; Occhipinti, Angelo; Reichert, Andreas S.; Terzic, Janos; Dötsch, Volker; Ney, Paul A.; Dikic, Ivan.

In: EMBO Reports, Vol. 11, No. 1, 01.01.2010, p. 45-51.

Research output: Contribution to journalArticle

Novak, I, Kirkin, V, McEwan, DG, Zhang, J, Wild, P, Rozenknop, A, Rogov, V, Löhr, F, Popovic, D, Occhipinti, A, Reichert, AS, Terzic, J, Dötsch, V, Ney, PA & Dikic, I 2010, 'Nix is a selective autophagy receptor for mitochondrial clearance', EMBO Reports, vol. 11, no. 1, pp. 45-51. https://doi.org/10.1038/embor.2009.256
Novak I, Kirkin V, McEwan DG, Zhang J, Wild P, Rozenknop A et al. Nix is a selective autophagy receptor for mitochondrial clearance. EMBO Reports. 2010 Jan 1;11(1):45-51. https://doi.org/10.1038/embor.2009.256
Novak, Ivana ; Kirkin, Vladimir ; McEwan, David G. ; Zhang, Ji ; Wild, Philipp ; Rozenknop, Alexis ; Rogov, Vladimir ; Löhr, Frank ; Popovic, Doris ; Occhipinti, Angelo ; Reichert, Andreas S. ; Terzic, Janos ; Dötsch, Volker ; Ney, Paul A. ; Dikic, Ivan. / Nix is a selective autophagy receptor for mitochondrial clearance. In: EMBO Reports. 2010 ; Vol. 11, No. 1. pp. 45-51.
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