NIX is required for programmed mitochondrial clearance during reticulocyte maturation

Rachel L. Schweers, Ji Zhang, Mindy S. Randall, Melanie R. Loyd, Weimin Li, Frank C. Dorsey, Mondira Kundu, Joseph T. Opferman, John L. Cleveland, Jeffery L. Miller, Paul A. Ney

Research output: Contribution to journalArticle

487 Citations (Scopus)

Abstract

The regulated clearance of mitochondria is a well recognized but poorly understood aspect of cellular homeostasis, and defects in this process have been linked to aging, degenerative diseases, and cancer. Mitochondria are recycled through an autophagy-related process, and reticulocytes, which completely eliminate their mitochondria during maturation, provide a physiological model to study this phenomenon. Here, we show that mitochondrial clearance in reticulocytes requires the BCL2-related protein NIX (BNIP3L). Mitochondrial clearance does not require BAX, BAK, BCL-XL, BIM, or PUMA, indicating that NIX does not function through established proapoptotic pathways. Similarly, NIX is not required for the induction of autophagy during terminal erythroid differentiation. NIX is required for the selective elimination of mitochondria, however, because mitochondrial clearance, in the absence of NIX, is arrested at the stage of mitochondrial incorporation into autophagosomes and autophagosome maturation. These results yield insight into the mechanism of mitochondrial clearance in higher eukaryotes. Furthermore, they show a BAX- and BAK-independent role for a BCL2-related protein in development.

Original languageEnglish (US)
Pages (from-to)19500-19505
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number49
DOIs
StatePublished - Dec 4 2007

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Reticulocytes
Proto-Oncogene Proteins c-bcl-2
Mitochondria
Autophagy
Eukaryota
Homeostasis
Neoplasms
Autophagosomes

Keywords

  • Autophagy
  • BCL2 family
  • Mitochondria

ASJC Scopus subject areas

  • General

Cite this

NIX is required for programmed mitochondrial clearance during reticulocyte maturation. / Schweers, Rachel L.; Zhang, Ji; Randall, Mindy S.; Loyd, Melanie R.; Li, Weimin; Dorsey, Frank C.; Kundu, Mondira; Opferman, Joseph T.; Cleveland, John L.; Miller, Jeffery L.; Ney, Paul A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 49, 04.12.2007, p. 19500-19505.

Research output: Contribution to journalArticle

Schweers, RL, Zhang, J, Randall, MS, Loyd, MR, Li, W, Dorsey, FC, Kundu, M, Opferman, JT, Cleveland, JL, Miller, JL & Ney, PA 2007, 'NIX is required for programmed mitochondrial clearance during reticulocyte maturation', Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 49, pp. 19500-19505. https://doi.org/10.1073/pnas.0708818104
Schweers, Rachel L. ; Zhang, Ji ; Randall, Mindy S. ; Loyd, Melanie R. ; Li, Weimin ; Dorsey, Frank C. ; Kundu, Mondira ; Opferman, Joseph T. ; Cleveland, John L. ; Miller, Jeffery L. ; Ney, Paul A. / NIX is required for programmed mitochondrial clearance during reticulocyte maturation. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 49. pp. 19500-19505.
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