No retroviremia or pathology in long-term follow-up of monkeys exposed to a murine amphotropic retrovirus

Kenneth Cornetta, Richard A. Morgan, Al Gillio, Sabine Sturm, Leon Baltrucki, Richard O'Reilly, W. French Anderson

Research output: Contribution to journalArticle

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Abstract

Four monkeys were exposed to a retroviral vector and replication-competent murine amphotropic retrovirus in a bone marrow transplantation/gene transfer protocol (Kantoff et al., 1987). We have studied these animals 2 and 3 years post-transplantation and did not detect replicating virus in serum, peripheral blood mononuclear cells, or bone marrow cells. Amphotropic envelope sequences could not be detected in blood or bone marrow cells by Southern blotting or the polymerase chain reaction. Antibodies directed against the p30 and gp70 viral antigens were detected by Western blot and immunoprecipitation. The animals remain alive and well. Our findings suggest that primates can clear murine amphotropic retroviruses even when exposure occurs during a time of severe immunosuppression.

Original languageEnglish
Pages (from-to)215-219
Number of pages5
JournalHuman Gene Therapy
Volume2
Issue number3
StatePublished - 1991

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Retroviridae
Bone Marrow Cells
Haplorhini
Pathology
Viral Antigens
Southern Blotting
Bone Marrow Transplantation
Immunoprecipitation
Immunosuppression
Primates
Blood Cells
Transplantation
Western Blotting
Viruses
Polymerase Chain Reaction
Antibodies
Serum
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Cornetta, K., Morgan, R. A., Gillio, A., Sturm, S., Baltrucki, L., O'Reilly, R., & Anderson, W. F. (1991). No retroviremia or pathology in long-term follow-up of monkeys exposed to a murine amphotropic retrovirus. Human Gene Therapy, 2(3), 215-219.

No retroviremia or pathology in long-term follow-up of monkeys exposed to a murine amphotropic retrovirus. / Cornetta, Kenneth; Morgan, Richard A.; Gillio, Al; Sturm, Sabine; Baltrucki, Leon; O'Reilly, Richard; Anderson, W. French.

In: Human Gene Therapy, Vol. 2, No. 3, 1991, p. 215-219.

Research output: Contribution to journalArticle

Cornetta, K, Morgan, RA, Gillio, A, Sturm, S, Baltrucki, L, O'Reilly, R & Anderson, WF 1991, 'No retroviremia or pathology in long-term follow-up of monkeys exposed to a murine amphotropic retrovirus', Human Gene Therapy, vol. 2, no. 3, pp. 215-219.
Cornetta, Kenneth ; Morgan, Richard A. ; Gillio, Al ; Sturm, Sabine ; Baltrucki, Leon ; O'Reilly, Richard ; Anderson, W. French. / No retroviremia or pathology in long-term follow-up of monkeys exposed to a murine amphotropic retrovirus. In: Human Gene Therapy. 1991 ; Vol. 2, No. 3. pp. 215-219.
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