No retroviremia or pathology in long-term follow-up of monkeys exposed to a murine amphotropic retrovirus

Kenneth Cornetta, Richard A. Morgan, Al Gillio, Sabine Sturm, Leon Baltrucki, Richard O'Reilly, W. French Anderson

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

Four monkeys were exposed to a retroviral vector and replication-competent murine amphotropic retrovirus in a bone marrow transplantation/gene transfer protocol (Kantoff et al., 1987). We have studied these animals 2 and 3 years post-transplantation and did not detect replicating virus in serum, peripheral blood mononuclear cells, or bone marrow cells. Amphotropic envelope sequences could not be detected in blood or bone marrow cells by Southern blotting or the polymerase chain reaction. Antibodies directed against the p30 and gp70 viral antigens were detected by Western blot and immunoprecipitation. The animals remain alive and well. Our findings suggest that primates can clear murine amphotropic retroviruses even when exposure occurs during a time of severe immunosuppression.

Original languageEnglish (US)
Pages (from-to)215-219
Number of pages5
JournalHuman gene therapy
Volume2
Issue number3
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

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