Nocardioides endophyticus sp. nov. and Nocardioides conyzicola sp. nov., isolated from herbaceous plant roots

Ji Hye Han, Tae Su Kim, Yochan Joung, Mi Na Kim, Kee Sun Shin, Taeok Bae, Seung Bum Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Two Gram-stain-positive, non-motile, non-spore-forming, rod-shaped actinobacterial strains were isolated from the surface-sterilized roots of mugwort (Artemisia princeps) and horse-weed (Conyza canadensis), and subjected to taxonomic characterization. 16S rRNA gene sequence analysis indicated that the isolates, designated MWE 3-5T and HWE 2-02T, should be placed in the genus Nocardioides of the family Nocardioidaceae. The strains were closely related to Nocardioides hankookensis DS-30T, which exhibited 16S rRNA gene sequence similarity values of 97.99 and 99.09 % with strains MWE 3-5T and HWE 2-02T, respectively. The genome relatedness of N. hankookensis DS-30T with strain MWE 3-5T was 35.8 %, and that with strain HWE 2-02T was 36.4 %, whereas that between the two isolates was 43.2 %. Strains MWE 3-5T and HWE 2-02T possessed MK-8(H4) as the major isoprenoid quinone, and LL-diaminopimelic acid in the cell-wall peptidoglycan. The main fatty acids were iso-C16: 0, iso-C17: 0 and C18: 1ω9c for strain MWE 3-5T and iso-C16: 0, 10-methyl C18: 0 and C18: 1ω9c for strain HWE 2-02T. Based on phenotypic, genotypic and phylogenetic studies, the following two novel species are proposed: Nocardioides endophyticus sp. nov. (type strain, MWE 3-5T = KCTC 29122T = JCM 18532T) and Nocardioides conyzicola sp. nov. (type strain, HWE 2-02T = KCTC 29121T = JCM 18531T).

Original languageEnglish (US)
Pages (from-to)4730-4734
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue numberPART 12
DOIs
StatePublished - Dec 1 2013

Fingerprint

Nocardioides
Artemisia
Plant Roots
herbaceous plants
rRNA Genes
herb
Conyza
Diaminopimelic Acid
Peptidoglycan
gene
isoprenoid
new species
Terpenes
horse
relatedness
Cell Wall
Horses
weed
Sequence Analysis
Fatty Acids

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Nocardioides endophyticus sp. nov. and Nocardioides conyzicola sp. nov., isolated from herbaceous plant roots. / Han, Ji Hye; Kim, Tae Su; Joung, Yochan; Kim, Mi Na; Shin, Kee Sun; Bae, Taeok; Kim, Seung Bum.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 63, No. PART 12, 01.12.2013, p. 4730-4734.

Research output: Contribution to journalArticle

Han, Ji Hye ; Kim, Tae Su ; Joung, Yochan ; Kim, Mi Na ; Shin, Kee Sun ; Bae, Taeok ; Kim, Seung Bum. / Nocardioides endophyticus sp. nov. and Nocardioides conyzicola sp. nov., isolated from herbaceous plant roots. In: International Journal of Systematic and Evolutionary Microbiology. 2013 ; Vol. 63, No. PART 12. pp. 4730-4734.
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abstract = "Two Gram-stain-positive, non-motile, non-spore-forming, rod-shaped actinobacterial strains were isolated from the surface-sterilized roots of mugwort (Artemisia princeps) and horse-weed (Conyza canadensis), and subjected to taxonomic characterization. 16S rRNA gene sequence analysis indicated that the isolates, designated MWE 3-5T and HWE 2-02T, should be placed in the genus Nocardioides of the family Nocardioidaceae. The strains were closely related to Nocardioides hankookensis DS-30T, which exhibited 16S rRNA gene sequence similarity values of 97.99 and 99.09 {\%} with strains MWE 3-5T and HWE 2-02T, respectively. The genome relatedness of N. hankookensis DS-30T with strain MWE 3-5T was 35.8 {\%}, and that with strain HWE 2-02T was 36.4 {\%}, whereas that between the two isolates was 43.2 {\%}. Strains MWE 3-5T and HWE 2-02T possessed MK-8(H4) as the major isoprenoid quinone, and LL-diaminopimelic acid in the cell-wall peptidoglycan. The main fatty acids were iso-C16: 0, iso-C17: 0 and C18: 1ω9c for strain MWE 3-5T and iso-C16: 0, 10-methyl C18: 0 and C18: 1ω9c for strain HWE 2-02T. Based on phenotypic, genotypic and phylogenetic studies, the following two novel species are proposed: Nocardioides endophyticus sp. nov. (type strain, MWE 3-5T = KCTC 29122T = JCM 18532T) and Nocardioides conyzicola sp. nov. (type strain, HWE 2-02T = KCTC 29121T = JCM 18531T).",
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AU - Shin, Kee Sun

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