Nodal signaling from the visceral endoderm is required to maintain Nodal gene expression in the epiblast and drive DVE/AVE migration

Amit Kumar, Margaret Lualdi, George T. Lyozin, Prashant Sharma, Jadranka Loncarek, Xin Yuan Fu, Michael R. Kuehn

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In the early mouse embryo, a specialized population of extraembryonic visceral endoderm (VE) cells called the distal VE (DVE) arises at the tip of the egg cylinder stage embryo and then asymmetrically migrates to the prospective anterior, recruiting additional distal cells. Upon migration these cells, called the anterior VE (AVE), establish the anterior posterior (AP) axis by restricting gastrulation-inducing signals to the opposite pole. The Nodal-signaling pathway has been shown to have a critical role in the generation and migration of the DVE/AVE. The Nodal gene is expressed in both the VE and in the pluripotent epiblast, which gives rise to the germ layers. Previous findings have provided conflicting evidence as to the relative importance of Nodal signaling from the epiblast vs. VE for AP patterning. Here we show that conditional mutagenesis of the Nodal gene specifically within the VE leads to reduced Nodal expression levels in the epiblast and incomplete or failed DVE/AVE migration. These results support a required role for VE Nodal to maintain normal levels of expression in the epiblast, and suggest signaling from both VE and epiblast is important for DVE/AVE migration.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalDevelopmental Biology
Volume400
Issue number1
DOIs
StatePublished - Apr 1 2015
Externally publishedYes

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Germ Layers
Endoderm
Gene Expression
Embryonic Structures
Gastrulation
Mutagenesis
Genes
Cell Movement
Ovum

Keywords

  • DVE/AVE
  • Epiblast
  • Nodal signaling
  • Ttr-Cre
  • Visceral endoderm

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Nodal signaling from the visceral endoderm is required to maintain Nodal gene expression in the epiblast and drive DVE/AVE migration. / Kumar, Amit; Lualdi, Margaret; Lyozin, George T.; Sharma, Prashant; Loncarek, Jadranka; Fu, Xin Yuan; Kuehn, Michael R.

In: Developmental Biology, Vol. 400, No. 1, 01.04.2015, p. 1-9.

Research output: Contribution to journalArticle

Kumar, Amit ; Lualdi, Margaret ; Lyozin, George T. ; Sharma, Prashant ; Loncarek, Jadranka ; Fu, Xin Yuan ; Kuehn, Michael R. / Nodal signaling from the visceral endoderm is required to maintain Nodal gene expression in the epiblast and drive DVE/AVE migration. In: Developmental Biology. 2015 ; Vol. 400, No. 1. pp. 1-9.
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