Non-overlapping functions for Pyk2 and FAK in osteoblasts during fluid shear stress-induced mechanotransduction

Suzanne R L Young, Julia M. Hum, Eric Rodenberg, Charles H. Turner, Fredrick Pavalko

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Mechanotransduction, the process by which cells convert external mechanical stimuli such as fluid shear stress (FSS) into biochemical changes, plays a critical role in maintenance of the skeleton. We have proposed that mechanical stimulation by FSS across the surfaces of bone cells results in formation of unique signaling complexes called mechanosomes that are launched from sites of adhesion with the extracellular matrix and with other bone cells [1]. Deformation of adhesion complexes at the cell membrane ultimately results in alteration of target gene expression. Recently, we reported that focal adhesion kinase (FAK) functions as a part of a mechanosome complex that is required for FSS-induced mechanotransduction in bone cells. This study extends this work to examine the role of a second member of the FAK family of non-receptor protein tyrosine kinases, proline-rich tyrosine kinase 2 (Pyk2), and determine its role during osteoblast mechanotransduction. We use osteoblasts harvested from mice as our model system in this study and compared the contributions of Pyk2 and FAK during FSS induced mechanotransduction in osteoblasts. We exposed Pyk2+/+ and Pyk2-/- primary calvarial osteoblasts to short period of oscillatory fluid flow and analyzed downstream activation of ERK1/2, and expression of c-fos, cyclooxygenase-2 and osteopontin. Unlike FAK, Pyk2 was not required for fluid flow-induced mechanotransduction as there was no significant difference in the response of Pyk2+/+ and Pyk2-/- osteoblasts to short periods of fluid flow (FF). In contrast, and as predicted, FAK-/- osteoblasts were unable to respond to FF. These data indicate that FAK and Pyk2 have distinct, non-redundant functions in launching mechanical signals during osteoblast mechanotransduction. Additionally, we compared two methods of generating FF in both cell types, oscillatory pump method and another orbital platform method. We determined that both methods of generating FF induced similar responses in both primary calvarial osteoblasts and immortalized calvarial osteoblasts.

Original languageEnglish
Article numbere16026
JournalPLoS One
Volume6
Issue number1
DOIs
StatePublished - 2011

Fingerprint

Focal Adhesion Kinase 2
non-specific protein-tyrosine kinase
Focal Adhesion Protein-Tyrosine Kinases
osteoblasts
Osteoblasts
shear stress
tyrosine
proline
Shear stress
phosphotransferases (kinases)
Fluids
Flow of fluids
Bone
bones
Bone and Bones
cells
adhesion
Adhesion
fluids
mechanotransduction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Non-overlapping functions for Pyk2 and FAK in osteoblasts during fluid shear stress-induced mechanotransduction. / Young, Suzanne R L; Hum, Julia M.; Rodenberg, Eric; Turner, Charles H.; Pavalko, Fredrick.

In: PLoS One, Vol. 6, No. 1, e16026, 2011.

Research output: Contribution to journalArticle

Young, Suzanne R L ; Hum, Julia M. ; Rodenberg, Eric ; Turner, Charles H. ; Pavalko, Fredrick. / Non-overlapping functions for Pyk2 and FAK in osteoblasts during fluid shear stress-induced mechanotransduction. In: PLoS One. 2011 ; Vol. 6, No. 1.
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