Non-rigid registration of multiphoton microscopy images using B-splines

Kevin S. Lorenz, Paul Salama, Kenneth W. Dunn, Edward J. Delp

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Optical microscopy poses many challenges for digital image analysis. One particular challenge includes correction of image artifacts due to respiratory motion from specimens imaged in vivo. We describe a non-rigid registration method using B-splines to correct these motion artifacts. Current attempts at non-rigid medical image registration have typically involved only a single pair of images. Extending these techniques to an entire series of images, possibly comprising hundreds of images, is presented in this paper. Our method involves creating a uniform grid of control points across each image in a stack. Each control point is manipulated by optimizing a cost function consisting of two parts: a term to determine image similarity, and a term to evaluate deformation grid smoothness. This process is repeated for all images in the stack. Analysis is evaluated using block motion estimation and other visualization techniques.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2011
Subtitle of host publicationImage Processing
StatePublished - Jun 9 2011
EventMedical Imaging 2011: Image Processing - Lake Buena Vista, FL, United States
Duration: Feb 14 2011Feb 16 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherMedical Imaging 2011: Image Processing
CountryUnited States
CityLake Buena Vista, FL


  • b-splines
  • image registration
  • microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Lorenz, K. S., Salama, P., Dunn, K. W., & Delp, E. J. (2011). Non-rigid registration of multiphoton microscopy images using B-splines. In Medical Imaging 2011: Image Processing [796221] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7962).