Non-rigid registration of multiphoton microscopy images using B-splines

Kevin S. Lorenz, Paul Salama, Kenneth Dunn, Edward J. Delp

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Optical microscopy poses many challenges for digital image analysis. One particular challenge includes correction of image artifacts due to respiratory motion from specimens imaged in vivo. We describe a non-rigid registration method using B-splines to correct these motion artifacts. Current attempts at non-rigid medical image registration have typically involved only a single pair of images. Extending these techniques to an entire series of images, possibly comprising hundreds of images, is presented in this paper. Our method involves creating a uniform grid of control points across each image in a stack. Each control point is manipulated by optimizing a cost function consisting of two parts: a term to determine image similarity, and a term to evaluate deformation grid smoothness. This process is repeated for all images in the stack. Analysis is evaluated using block motion estimation and other visualization techniques.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7962
DOIs
StatePublished - 2011
EventMedical Imaging 2011: Image Processing - Lake Buena Vista, FL, United States
Duration: Feb 14 2011Feb 16 2011

Other

OtherMedical Imaging 2011: Image Processing
CountryUnited States
CityLake Buena Vista, FL
Period2/14/112/16/11

Fingerprint

splines
Splines
Microscopy
Microscopic examination
microscopy
Artifacts
Image registration
Motion estimation
Cost functions
Image analysis
Optical microscopy
Visualization
artifacts
Costs and Cost Analysis
grids
image analysis
costs

Keywords

  • b-splines
  • image registration
  • microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Lorenz, K. S., Salama, P., Dunn, K., & Delp, E. J. (2011). Non-rigid registration of multiphoton microscopy images using B-splines. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7962). [796221] https://doi.org/10.1117/12.878304

Non-rigid registration of multiphoton microscopy images using B-splines. / Lorenz, Kevin S.; Salama, Paul; Dunn, Kenneth; Delp, Edward J.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7962 2011. 796221.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lorenz, KS, Salama, P, Dunn, K & Delp, EJ 2011, Non-rigid registration of multiphoton microscopy images using B-splines. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7962, 796221, Medical Imaging 2011: Image Processing, Lake Buena Vista, FL, United States, 2/14/11. https://doi.org/10.1117/12.878304
Lorenz KS, Salama P, Dunn K, Delp EJ. Non-rigid registration of multiphoton microscopy images using B-splines. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7962. 2011. 796221 https://doi.org/10.1117/12.878304
Lorenz, Kevin S. ; Salama, Paul ; Dunn, Kenneth ; Delp, Edward J. / Non-rigid registration of multiphoton microscopy images using B-splines. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7962 2011.
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