Noncovalent keystone interactions controlling biomembrane structure

Roger G. Hanshaw, Robert Stahelin, Bradley D. Smith

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

There is a biomedical need to develop molecular recognition systems that selectively target the interfaces of protein and lipid aggregates in biomembranes. This is an extremely challenging problem in supramolecular chemistry because the biological membrane is a complex dynamic assembly of multifarious molecular components with local inhomogeneity. Two simplifying concepts are presented as a framework for basing molecular design strategies. The first generalization is that association of two binding partners in a biomembrane will be dominated by one type of non-covalent interaction which is referred to as the keystone interaction. Structural mutations in membrane proteins that alter the strength of this keystone interaction will likely have a major effect on biological activity and often will be associated with disease. The second generalization is to view the structure of a cell membrane as three spatial regions, that is, the polar membrane surface, the midpolar interfacial region and the non-polar membrane interior. Each region has a distinct dielectric, and the dominating keystone interaction between binding partners will be different. At the highly polar membrane surface, the keystone interactions between charged binding partners are ion-ion and ion-dipole interactions; whereas, ion-dipole and ionic hydrogen bonding are very influential at the mid-polar interfacial region. In the non-polar membrane interior, van der Waals forces and neutral hydrogen bonding are the keystone interactions that often drive molecular association. Selected examples of lipid and transmembrane protein association systems are described to illustrate how the association thermodynamics and kinetics are dominated by these keystone noncovalent interactions.

Original languageEnglish
Pages (from-to)1690-1697
Number of pages8
JournalChemistry (Weinheim an der Bergstrasse, Germany)
Volume14
Issue number6
DOIs
StatePublished - Feb 18 2008

Fingerprint

Association reactions
Ions
Membranes
Hydrogen bonds
Proteins
Supramolecular chemistry
Lipids
Biological membranes
Molecular recognition
Van der Waals forces
Cell membranes
Bioactivity
Membrane Proteins
Thermodynamics
Kinetics

Keywords

  • Hydrogen bonds
  • Ion-dipole interactions
  • Membranes
  • Molecular recognition
  • Phospholipids

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Noncovalent keystone interactions controlling biomembrane structure. / Hanshaw, Roger G.; Stahelin, Robert; Smith, Bradley D.

In: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 14, No. 6, 18.02.2008, p. 1690-1697.

Research output: Contribution to journalArticle

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