Nonexpression of the human serum amyloid A three (SAA3) gene

B. Kluve-Beckerman, M. L. Drumm, Merrill Benson

Research output: Contribution to journalArticle

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Abstract

Serum amyloid A (SAA) is a major acute-phase plasma protein synthesized by the liver. In addition to the two major plasma isoforms described in humans (SAA1 and SAA2), a third form (SAA3) has been demonstrated in several other species and is distinguished by predominant extrahepatic expression. Two clones, Ch11g5-1-1 and HDg1-1, containing the human SAA3 gene are described in this report. The human SAA3 gene is comparable in organization to the SAA1 and SAA2 genes and shares with them 87% nucleotide identity in the region spanning exon 3 through exon 4. Sequences 5' to exon 3, however, are strikingly different from those in the SAA1 and SAA2 genes. For instance, the sequence deduced for amino acids 1-12 (exon 2) has only 25% identity with human SAA1 and SAA2; it most closely resembles that of rabbit SAA3 isolated from synovial fibroblast cultures (75% identity). Although rabbit SAA3 induces collagenase production in an autocrine fashion the human SAA3 gene is not expressed. This is shown by: (i) a single base insertion in the sequence corresponding to codon 31, (ii) the inability of a 918-bp fragment immediately upstream from SAA3 exon sequences to direct transcription of a chloramphenicol acetyltransferase reporter gene, and (iii) the absence of detectable human SAA3 in mRNA.

Original languageEnglish (US)
Pages (from-to)651-661
Number of pages11
JournalDNA and Cell Biology
Volume10
Issue number9
StatePublished - 1991

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Serum Amyloid A Protein
Exons
Genes
Rabbits
Chloramphenicol O-Acetyltransferase
Acute-Phase Proteins
Insertional Mutagenesis
Collagenases
Reporter Genes
Codon
Blood Proteins
Amino Acid Sequence
Protein Isoforms
Nucleotides
Clone Cells
Fibroblasts
Messenger RNA
Liver

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Nonexpression of the human serum amyloid A three (SAA3) gene. / Kluve-Beckerman, B.; Drumm, M. L.; Benson, Merrill.

In: DNA and Cell Biology, Vol. 10, No. 9, 1991, p. 651-661.

Research output: Contribution to journalArticle

Kluve-Beckerman, B, Drumm, ML & Benson, M 1991, 'Nonexpression of the human serum amyloid A three (SAA3) gene', DNA and Cell Biology, vol. 10, no. 9, pp. 651-661.
Kluve-Beckerman, B. ; Drumm, M. L. ; Benson, Merrill. / Nonexpression of the human serum amyloid A three (SAA3) gene. In: DNA and Cell Biology. 1991 ; Vol. 10, No. 9. pp. 651-661.
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