Nonmarrow hematopoiesis occurs in a hyaluronic-acid-rich node and duct system in mice

Sunhee Hwang, Seung J. Lee, Sang H. Park, Brahmananda R. Chitteti, Edward F. Srour, Scott Cooper, Giao Hangoc, Hal E. Broxmeyer, Byoung S. Kwon

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A hyaluronic-acid-rich node and duct system (HAR-NDS) was found on the surface of internal organs of mice, and inside their blood and lymph vessels. The nodes (HAR-Ns) were filled with immune cells of the innate system and were especially enriched with mast cells and histiocytes. They also contained hematopoietic progenitor cells (HPCs), such as granulocyte-macrophage, erythroid, multipotential progenitors, and mast cell progenitors (MCPs). MCPs were the most abundant among the HPCs in HAR-Ns. Their frequency was fivefold higher than that of the MCPs in bone marrow. In addition, the system contained pluripotent stem cells (PSCs) capable of producing CD45-Flk1+ hemangioblast-like cells, which subsequently generated various types of HPCs and differentiated blood cells. Although HAR-Ns did not appear to harbor enough number of cells capable of long-term reconstitution or short-term radioprotection of lethally irradiated recipients, bone marrow cells were able to engraft in the HAR-NDS and reconstitute hematopoietic potentials of the system. PSCs and HPCs were consistently found in intravenous, intralymphatic, and intestinal HAR-ND. We infer that PSCs and HPCs reside in the HAR-ND and that this novel system may serve as an alternative means to traffic immature and mature blood cells throughout the body.

Original languageEnglish (US)
Pages (from-to)2661-2671
Number of pages11
JournalStem cells and development
Volume23
Issue number21
DOIs
StatePublished - Nov 1 2014

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

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    Hwang, S., Lee, S. J., Park, S. H., Chitteti, B. R., Srour, E. F., Cooper, S., Hangoc, G., Broxmeyer, H. E., & Kwon, B. S. (2014). Nonmarrow hematopoiesis occurs in a hyaluronic-acid-rich node and duct system in mice. Stem cells and development, 23(21), 2661-2671. https://doi.org/10.1089/scd.2014.0075