Nonobese diabetic mice have diminished gallbladder motility and shortened crystal observation time

Shannon J. Graewin, James M. Kiely, Keun Ho Lee, Carol L. Svatek, Attila Nakeeb, Henry A. Pitt, Thomas Magnuson, Frank Moody

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Diabetes and obesity are strongly associated and are risk factors for cholesterol gallstone disease. Leptin-deficient and leptin-resistant diabetic obese mice have enlarged, hypomotile gallbladders. In addition, bile from gallbladders of leptin-deficient mice has enhanced cholesterol crystal formation, whereas bile from gallbladders of leptin-resistant mice has delayed crystal observation time. To determine the effect of diabetes alone, we hypothesized that leptin-normal, nonobese diabetic (NOD) mice would have reduced biliary motility and rapid crystal formation. Twenty control and 9 prediabetic and 11 diabetic NOD, 12- to 26-week-old mice underwent glucose measurement and cholecystectomy for muscle bath stimulation with neurotransmitters. An additional group of 200 control and 78 NOD 12-week-old mice underwent microscopic bile examination for cholesterol crystal formation. Compared with control mice, prediabetic NOD mice had similar glucose levels and gallbladder volumes. Diabetic NOD mice had higher sugar levels and larger gallbladder volumes (P <0.001) than control mice. Prediabetic NOD gallbladders had less contractility (P <0.01) than control gallbladders, and contractility worsened (P <0.01) in diabetic NOD mice. NOD mice formed cholesterol crystals earlier than did control mice (P <0.05). Nonobese diabetic NOD mice have (1) decreased gallbladder contraction to neurotransmitters, which worsens with development of diabetes, and (2) rapid crystal formation. We conclude that diabetes alone alters gallbladder motility and cholesterol crystal formation.

Original languageEnglish (US)
Pages (from-to)824-830
Number of pages7
JournalJournal of Gastrointestinal Surgery
Volume8
Issue number7
DOIs
StatePublished - 2004
Externally publishedYes

Fingerprint

Inbred NOD Mouse
Gallbladder
Observation
Leptin
Cholesterol
Bile
Neurotransmitter Agents
Glucose
Obese Mice
Cholecystectomy
Gallstones
Baths
Type 2 Diabetes Mellitus
Obesity
Muscles
Control Groups

Keywords

  • Cholesterol
  • Diabetes mellitus
  • Gallbladder
  • Gallstones
  • Motility

ASJC Scopus subject areas

  • Surgery

Cite this

Nonobese diabetic mice have diminished gallbladder motility and shortened crystal observation time. / Graewin, Shannon J.; Kiely, James M.; Lee, Keun Ho; Svatek, Carol L.; Nakeeb, Attila; Pitt, Henry A.; Magnuson, Thomas; Moody, Frank.

In: Journal of Gastrointestinal Surgery, Vol. 8, No. 7, 2004, p. 824-830.

Research output: Contribution to journalArticle

Graewin, Shannon J. ; Kiely, James M. ; Lee, Keun Ho ; Svatek, Carol L. ; Nakeeb, Attila ; Pitt, Henry A. ; Magnuson, Thomas ; Moody, Frank. / Nonobese diabetic mice have diminished gallbladder motility and shortened crystal observation time. In: Journal of Gastrointestinal Surgery. 2004 ; Vol. 8, No. 7. pp. 824-830.
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