Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol

Brittney Shea Herbert, Amelia E. Hochreiter, Woodring E. Wright, Jerry W. Shay

Research output: Contribution to journalArticle

152 Scopus citations

Abstract

The telomeric repeat amplification protocol (TRAP) is a two-step process for analyzing telomerase activity in cell or tissue extracts. Recent modifications of this sensitive assay include elimination of radioactivity by using a fluorescently labeled primer instead of a radiolabeled primer. In addition, the TRAP assay has been modified for real-time, quantitative PCR analysis. Here, we describe cost-effective procedures for detection of telomerase activity using a fluorescent-based assay as well as by using real-time PCR. These modified TRAP assays can be accomplished within 4 h (from lysis of samples to analysis of telomerase products).

Original languageEnglish (US)
Pages (from-to)1583-1590
Number of pages8
JournalNature protocols
Volume1
Issue number3
DOIs
StatePublished - Aug 1 2006

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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