Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol

Brittney-Shea Herbert, Amelia E. Hochreiter, Woodring E. Wright, Jerry W. Shay

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

The telomeric repeat amplification protocol (TRAP) is a two-step process for analyzing telomerase activity in cell or tissue extracts. Recent modifications of this sensitive assay include elimination of radioactivity by using a fluorescently labeled primer instead of a radiolabeled primer. In addition, the TRAP assay has been modified for real-time, quantitative PCR analysis. Here, we describe cost-effective procedures for detection of telomerase activity using a fluorescent-based assay as well as by using real-time PCR. These modified TRAP assays can be accomplished within 4 h (from lysis of samples to analysis of telomerase products).

Original languageEnglish
Pages (from-to)1583-1590
Number of pages8
JournalNature Protocols
Volume1
Issue number3
DOIs
StatePublished - Aug 2006

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Telomerase
Amplification
Assays
Real-Time Polymerase Chain Reaction
Tissue Extracts
Cell Extracts
Radioactivity
Costs and Cost Analysis
Chemical analysis
Costs

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol. / Herbert, Brittney-Shea; Hochreiter, Amelia E.; Wright, Woodring E.; Shay, Jerry W.

In: Nature Protocols, Vol. 1, No. 3, 08.2006, p. 1583-1590.

Research output: Contribution to journalArticle

Herbert, Brittney-Shea ; Hochreiter, Amelia E. ; Wright, Woodring E. ; Shay, Jerry W. / Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol. In: Nature Protocols. 2006 ; Vol. 1, No. 3. pp. 1583-1590.
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