Noradrenaline modulates calcium channels in avian dorsal root ganglion cells through tight receptor‐channel coupling.

P. Forscher, G. S. Oxford, D. Schulz

Research output: Contribution to journalArticle

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Abstract

Averaged ensemble Ba currents were recorded from tissue cultured embryonic chick dorsal root ganglion (d.r.g.) cells using the cell‐attached patch‐clamp technique. Noradrenaline (NA) applied to extrapatch membrane had no clear consistent effect on drug‐free patch currents. This finding supports a previous suggestion that second messengers may not be involved in NA‐mediated decreases in Ca currents in sensory neurones (Forscher & Oxford, 1985). Cell‐attached patch currents sometimes increased slowly after extrapatch application of NA, but were not reversibly decreased by drug treatment. Large patch currents were used to trigger cellular action potentials. NA reversibly decreased action potential duration as reflected in extracellularly recorded patch action currents. Simultaneously recorded inward patch currents were not affected. D.r.g. cell adenylate cyclase activity was assayed. NA did not affect intracellular cyclic AMP levels at concentrations which cause 30‐70% decreases in gCa in dialysed cells (Forscher & Oxford, 1985). Treatment with forskolin (50 microM) or isoprenaline (10 microM) resulted in 60‐ and 2‐fold increases respectively in adenylate cyclase activity over basal levels. These results suggest that NA decreases Ca currents by direct NA interactions with the Ca channel or a molecule tightly coupled to channel function in d.r.g. cells.

Original languageEnglish (US)
Pages (from-to)131-144
Number of pages14
JournalJournal of Physiology
Volume379
Issue number1
DOIs
StatePublished - Oct 1 1986
Externally publishedYes

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Spinal Ganglia
Calcium Channels
Norepinephrine
Adenylyl Cyclases
Action Potentials
Second Messenger Systems
Sensory Receptor Cells
Colforsin
Isoproterenol
Cyclic AMP
Membranes
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Physiology

Cite this

Noradrenaline modulates calcium channels in avian dorsal root ganglion cells through tight receptor‐channel coupling. / Forscher, P.; Oxford, G. S.; Schulz, D.

In: Journal of Physiology, Vol. 379, No. 1, 01.10.1986, p. 131-144.

Research output: Contribution to journalArticle

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