Normal neutrophil function in human renal allograft recipients receiving Minnesota anti-lymphoblast globulin (MAG)

D. A. Hawley, G. A. Miller, J. L. Biesecker

Research output: Contribution to journalArticle

Abstract

Equine anti-human lymphoblast globulin (MAG) used in combination with other immunosuppressive agents did not impair chemotaxis, phagocytosis, nitroblue tetrazolium (NBT) reduction or halogenation by peripheral blood neutrophils (PMN) from seven human recipients of cadaver renal allografts. MAG was given to the allograft recipients following transplantation, and the dosage was determined each day by quantitation of peripheral blood T and B lymphocytes. No deposition of immunoglobulin or complement on the recipient's PMN was detected by indirect immunofluorescence. Chemotaxis of PMN was determined in blind-well chambers, and phagocytosis of opsonized zymosan was quantitated by light microscopy. Reduction of NBT was quantitated by spectrophotometric measurement of solubilized formazan, and halogenation was measured by quantitating the incorporation of 125I into PMN that had phagocytosed opsonized zymosan. Chemotaxis, phagocytosis, NBT reduction, and halogenation by neutrophils from patients receiving MAG, prednisone and azathioprine were not impaired.

Original languageEnglish
Pages (from-to)29-33
Number of pages5
JournalAmerican Journal of Clinical Pathology
Volume76
Issue number1
StatePublished - 1981

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Globulins
Nitroblue Tetrazolium
Phagocytosis
Allografts
Halogenation
Neutrophils
Chemotaxis
Kidney
Zymosan
Formazans
Azathioprine
Immunosuppressive Agents
Indirect Fluorescent Antibody Technique
Prednisone
Cadaver
Horses
Immunoglobulins
Microscopy
B-Lymphocytes
Transplantation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Normal neutrophil function in human renal allograft recipients receiving Minnesota anti-lymphoblast globulin (MAG). / Hawley, D. A.; Miller, G. A.; Biesecker, J. L.

In: American Journal of Clinical Pathology, Vol. 76, No. 1, 1981, p. 29-33.

Research output: Contribution to journalArticle

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AB - Equine anti-human lymphoblast globulin (MAG) used in combination with other immunosuppressive agents did not impair chemotaxis, phagocytosis, nitroblue tetrazolium (NBT) reduction or halogenation by peripheral blood neutrophils (PMN) from seven human recipients of cadaver renal allografts. MAG was given to the allograft recipients following transplantation, and the dosage was determined each day by quantitation of peripheral blood T and B lymphocytes. No deposition of immunoglobulin or complement on the recipient's PMN was detected by indirect immunofluorescence. Chemotaxis of PMN was determined in blind-well chambers, and phagocytosis of opsonized zymosan was quantitated by light microscopy. Reduction of NBT was quantitated by spectrophotometric measurement of solubilized formazan, and halogenation was measured by quantitating the incorporation of 125I into PMN that had phagocytosed opsonized zymosan. Chemotaxis, phagocytosis, NBT reduction, and halogenation by neutrophils from patients receiving MAG, prednisone and azathioprine were not impaired.

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