Novel bioactive tetracycline-containing electrospun polymer fibers as a potential antibacterial dental implant coating

R. G. Shahi, M. T P Albuquerque, E. A. Münchow, Steven Blanchard, Richard Gregory, M. C. Bottino

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The purpose of this investigation was to determine the ability of tetracycline-containing fibers to inhibit biofilm formation of peri-implantitis-associated pathogens [i.e., Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), Prevotella intermedia (Pi), and Aggregatibacter actinomycetemcomitans (Aa)]. Tetracycline hydrochloride (TCH) was added to a poly(DL-lactide) [PLA], poly(ε-caprolactone) [PCL], and gelatin [GEL] polymer blend solution at distinct concentrations to obtain the following fibers: PLA:PCL/GEL (TCH-free, control), PLA:PCL/GEL + 5 % TCH, PLA:PCL/GEL + 10 % TCH, and PLA:PCL/GEL + 25 % TCH. The inhibitory effect of TCH-containing fibers on biofilm formation was assessed by colony-forming units (CFU/mL). Qualitative analysis of biofilm inhibition was done via scanning electron microscopy (SEM). Statistical significance was reported at p < 0.05. Complete inhibition of biofilm formation on the fibers was observed in groups containing TCH at 10 and 25 wt%. Fibers containing TCH at 5 wt% demonstrated complete inhibition of Aa biofilm. Even though a marked reduction in CFU/mL was observed with an increase in TCH concentration, Pi proved to be the most resilient microorganism. SEM images revealed the absence of or a notable decrease in bacterial biofilm on the TCH-containing nanofibers. Collectively, our data suggest that tetracycline-containing fibers hold great potential as an antibacterial dental implant coating.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalOdontology
DOIs
StateAccepted/In press - Sep 1 2016

Fingerprint

Dental Implants
Tetracycline
Polymers
Biofilms
Gelatin
Prevotella intermedia
Aggregatibacter actinomycetemcomitans
Electron Scanning Microscopy
Peri-Implantitis
Fusobacterium nucleatum
Nanofibers
Porphyromonas gingivalis
Stem Cells
polycaprolactone

Keywords

  • Coating
  • Implant
  • Nanofibers
  • Peri-implantitis
  • Tetracycline

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Novel bioactive tetracycline-containing electrospun polymer fibers as a potential antibacterial dental implant coating. / Shahi, R. G.; Albuquerque, M. T P; Münchow, E. A.; Blanchard, Steven; Gregory, Richard; Bottino, M. C.

In: Odontology, 01.09.2016, p. 1-10.

Research output: Contribution to journalArticle

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abstract = "The purpose of this investigation was to determine the ability of tetracycline-containing fibers to inhibit biofilm formation of peri-implantitis-associated pathogens [i.e., Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), Prevotella intermedia (Pi), and Aggregatibacter actinomycetemcomitans (Aa)]. Tetracycline hydrochloride (TCH) was added to a poly(DL-lactide) [PLA], poly(ε-caprolactone) [PCL], and gelatin [GEL] polymer blend solution at distinct concentrations to obtain the following fibers: PLA:PCL/GEL (TCH-free, control), PLA:PCL/GEL + 5 {\%} TCH, PLA:PCL/GEL + 10 {\%} TCH, and PLA:PCL/GEL + 25 {\%} TCH. The inhibitory effect of TCH-containing fibers on biofilm formation was assessed by colony-forming units (CFU/mL). Qualitative analysis of biofilm inhibition was done via scanning electron microscopy (SEM). Statistical significance was reported at p < 0.05. Complete inhibition of biofilm formation on the fibers was observed in groups containing TCH at 10 and 25 wt{\%}. Fibers containing TCH at 5 wt{\%} demonstrated complete inhibition of Aa biofilm. Even though a marked reduction in CFU/mL was observed with an increase in TCH concentration, Pi proved to be the most resilient microorganism. SEM images revealed the absence of or a notable decrease in bacterial biofilm on the TCH-containing nanofibers. Collectively, our data suggest that tetracycline-containing fibers hold great potential as an antibacterial dental implant coating.",
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