Novel chlamydia trachomatis strains in heterosexual sex partners, Indianapolis, Indiana, USA

Byron Batteiger, Raymond Wan, James A. Williams, Linda He, Arissa Ma, J. Fortenberry, Deborah Dean

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Chlamydia trachomatis causes a high number of sexually transmitted infections worldwide, but reproducible and precise strain typing to link partners is lacking. We evaluated multilocus sequence typing (MLST) for this purpose by detecting sequence types (STs) concordant for the ompA genotype, a single-locus typing standard. We tested samples collected during April 2000-October 2003 from members of established heterosexual partnerships (dyads) in the Indianapolis, Indiana, USA, area who self-reported being coital partners within the previous 30 days. C. trachomatis DNA from 28 dyads was tested by MLST; sequences were aligned and analyzed for ST and phylogenetic relationships. MLST detected 9 C. trachomatis STs, 4 unique to Indianapolis; STs were identical within each dyad. Thirteen unique strains were identified; 9 (32%) dyads harbored novel recombinant strains that phylogenetically clustered with strains comprising the recombinants. The high rate of novel C. trachomatis recombinants identified supports the use of MLST for transmission and strain diversity studies among at-risk populations.

Original languageEnglish (US)
Pages (from-to)1841-1847
Number of pages7
JournalEmerging Infectious Diseases
Volume20
Issue number11
DOIs
StatePublished - Nov 1 2014

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ASJC Scopus subject areas

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases

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