Novel E. coli mutants deficient in blosynthesis of 5-methylamlnomethyl-2-thiouridine

Drik Elseviers, Lynn A. Petrullo, Patricia Gallagher

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Novel E. coli mutants deficient in biosynthesis of 5-methylaminomethyl-2- thiouridine were isolated based on a phenotype of reduced readthrough at UAG codons. They define 2 new loci trmE and trmF, near 83′ on the E. coli map. These mutants are different from strains carrying trmC mutations, which are known to confer a methylation deficiency in biosynthesis of 5-methylaminomethyl-2-thiouridine. tRNA from mutants carrying trmE or trmF mutations was shown to carry 2-thiouridine instead of 5-methylaminomethyl-2-thiouridine. This deficiency affects the triplet binding properties of the mutant tRNA. Our results suggest that the 5-methylaminomethyl group stabilizes the basepairing this modified nucleotide with G, most likely through direct interaction with the ribosomal binding site(s).

Original languageEnglish (US)
Pages (from-to)3521-3534
Number of pages14
JournalNucleic Acids Research
Volume12
Issue number8
DOIs
StatePublished - Apr 25 1984
Externally publishedYes

Fingerprint

Biosynthesis
Mutant
Escherichia coli
Escherichia Coli
Transfer RNA
Methylation
Binding sites
Nucleotides
Mutation
Terminator Codon
Binding Sites
Phenotype
Locus
Likely
5-methylaminomethyl-2-thiouridine
2-thiouridine
Interaction

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Novel E. coli mutants deficient in blosynthesis of 5-methylamlnomethyl-2-thiouridine. / Elseviers, Drik; Petrullo, Lynn A.; Gallagher, Patricia.

In: Nucleic Acids Research, Vol. 12, No. 8, 25.04.1984, p. 3521-3534.

Research output: Contribution to journalArticle

Elseviers, Drik ; Petrullo, Lynn A. ; Gallagher, Patricia. / Novel E. coli mutants deficient in blosynthesis of 5-methylamlnomethyl-2-thiouridine. In: Nucleic Acids Research. 1984 ; Vol. 12, No. 8. pp. 3521-3534.
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