Novel indirect calorimetry technology to analyze metabolism in individual neonatal rodent pups

Jesus F. Dominguez, Lixin Guo, Marco A.Carrasco Molnar, Antonio Ballester Escobedo, Taylor Dunphy, Trent D. Lund, Jack Turman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: The ability to characterize the development of metabolic function in neonatal rodents has been limited due to technological constraints. Low respiratory volumes and flows at rest pose unique problems, making it difficult to reliably measure O2 consumption, CO2 production, respiratory quotient (RQ), and energy expenditure (EE). Our aim was to develop and validate a commercial-grade indirect calorimetry system capable of characterizing the metabolic phenotype of individual neonatal rodents. Methodology/Principal Findings: To address this research need, we developed a novel, highly sensitive open-circuit indirect calorimetry system capable of analyzing respiratory gas exchange in a single neonatal rodent pup. Additionally, we derived an equation from known metabolic relationships to estimate inlet flow rates, improving the efficiency of data collection. To validate the neonatal rodent indirect calorimetry system and evaluate the applicability of the derived equation for predicting appropriate flow rates, we conducted a series of experiments evaluating the impact of sex, litter size, time of day (during the light phase), and ambient temperature on neonatal rat metabolic parameters. Data revealed that the only metabolic parameter influenced by litter size is a neonatal rat's RQ, with rat pups reared in a small litter (5 pups) having lower RQ's than rat pups reared in either medium (8 pups) or large (11 pups) litters. Furthermore, data showed that ambient temperature affected all metabolic parameters measured, with colder temperatures being associated with higher CO2 production, higher O2 consumption, and higher energy expenditure. Conclusion/Significance: The results of this study demonstrate that the modified Panlab Oxylet system reliably assesses early postnatal metabolism in individual neonatal rodents. This system will be of paramount importance to further our understanding of processes associated with the developmental origins of adult metabolic disease.

Original languageEnglish (US)
Article numbere6790
JournalPLoS One
Volume4
Issue number8
DOIs
StatePublished - Aug 27 2009
Externally publishedYes

Fingerprint

Indirect Calorimetry
calorimetry
Calorimetry
Metabolism
pups
Rodentia
rodents
Rats
Technology
metabolism
Litter Size
respiratory quotient
Energy Metabolism
litters (young animals)
litter size
energy expenditure
Flow rate
ambient temperature
neonates
pulmonary gas exchange

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Dominguez, J. F., Guo, L., Molnar, M. A. C., Escobedo, A. B., Dunphy, T., Lund, T. D., & Turman, J. (2009). Novel indirect calorimetry technology to analyze metabolism in individual neonatal rodent pups. PLoS One, 4(8), [e6790]. https://doi.org/10.1371/journal.pone.0006790

Novel indirect calorimetry technology to analyze metabolism in individual neonatal rodent pups. / Dominguez, Jesus F.; Guo, Lixin; Molnar, Marco A.Carrasco; Escobedo, Antonio Ballester; Dunphy, Taylor; Lund, Trent D.; Turman, Jack.

In: PLoS One, Vol. 4, No. 8, e6790, 27.08.2009.

Research output: Contribution to journalArticle

Dominguez JF, Guo L, Molnar MAC, Escobedo AB, Dunphy T, Lund TD et al. Novel indirect calorimetry technology to analyze metabolism in individual neonatal rodent pups. PLoS One. 2009 Aug 27;4(8). e6790. https://doi.org/10.1371/journal.pone.0006790
Dominguez, Jesus F. ; Guo, Lixin ; Molnar, Marco A.Carrasco ; Escobedo, Antonio Ballester ; Dunphy, Taylor ; Lund, Trent D. ; Turman, Jack. / Novel indirect calorimetry technology to analyze metabolism in individual neonatal rodent pups. In: PLoS One. 2009 ; Vol. 4, No. 8.
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