Novel localization of a Na+/H+ exchanger in a late endosomal compartment of yeast

Implications for vacuole biogenesis

Richard Nass, Rajini Rao

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

Na+/H+ exchangers catalyze the electrically silent countertransport of Na+ and H+, controlling the transmembrane movement of salt, water, and acid-base equivalents, and are therefore critical for Na+ tolerance, cell volume control, and pH regulation. In contrast to numerous well studied plasma membrane isoforms (NHE1-4), much less is known about intracellular Na+/H+ exchangers, and thus far no vertebrate isoform has been shown to have an exclusively endosomal distribution. In this context, we show that the yeast NHE homologue, Nhx1 (Nass, R., Cunningham, K. W., and Rao, R. (1997) J. Biol. Chem. 272, 26145-26152), localizes uniquely to prevacuolar compartments, equivalent to late endosomes of animal cells. In living yeast, we show that these compartments closely abut the vacuolar membrane in a striking bipolar distribution, suggesting that vacuole biogenesis occurs at distinct sites. Nhx1 is the founding member of a newly emergent cluster of exchanger homologues, from yeasts, worms, and humans that may share a common intracellular localization. By compartmentalizing Na+, intracellular exchangers play an important role in halotolerance; furthermore, we hypothesize that salt and water movement into vesicles may regulate vesicle volume and pH and thus contribute to vacuole biogenesis.

Original languageEnglish (US)
Pages (from-to)21054-21060
Number of pages7
JournalJournal of Biological Chemistry
Volume273
Issue number33
DOIs
StatePublished - Aug 14 1998
Externally publishedYes

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Sodium-Hydrogen Antiporter
Vacuoles
Yeast
Water Movements
Yeasts
Protein Isoforms
Salts
Saline water
Endosomes
Cell membranes
Cell Size
Vertebrates
Animals
Cells
Cell Membrane
Membranes
Acids
Water

ASJC Scopus subject areas

  • Biochemistry

Cite this

Novel localization of a Na+/H+ exchanger in a late endosomal compartment of yeast : Implications for vacuole biogenesis. / Nass, Richard; Rao, Rajini.

In: Journal of Biological Chemistry, Vol. 273, No. 33, 14.08.1998, p. 21054-21060.

Research output: Contribution to journalArticle

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