Novel methods for determining hematopoietic stem and progenitor cell emergence in the murine yolk sac

Christopher T. Lux, Mervin C. Yoder

Research output: Contribution to journalReview article

5 Scopus citations

Abstract

The mammalian yolk sac is known to play a prominent role in emergence of the hematopoietic system. The extent of this contribution has been a subject of debate in recent years largely due to effects of the early circulation that obscures the site of origin of hematopoietic stem and progenitor cells. This review discusses the limitations of some of the standard assays currently employed to study hematopoietic stem and progenitor cell emergence and highlights several recently reported novel methods that address this problem from new perspectives. Two methods directly alter the circulation by either preventing it from occurring in the first place or by removing vascular connections between the embryo and the yolk sac. Other approaches have altered the ability of hematopoietic cells to interact with their environment, resulting in the lack of migration or an inability to bind to potential hematopoietic niches. A third set of experiments utilize lineage tracing techniques to follow the migration of early progenitors once they enter the circulation. Taken together, these novel methods provide new evidence for the contribution of yolk sac hematopoietic stem and progenitor cells to the adult hematopoietic system.

Original languageEnglish (US)
Pages (from-to)1003-1009
Number of pages7
JournalInternational Journal of Developmental Biology
Volume54
Issue number6-7
DOIs
StatePublished - Jul 28 2010

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Keywords

  • Embryogenesis
  • Hemogenic endothelium
  • Lineage tracing
  • Stromal co-culture
  • Transgenic mice

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology

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